New advances in proteomics, driven largely by developments in mass spectrometry, continue to reveal the complexity and diversity of pathogenic mechanisms among microbes that underpin infectious diseases. Therefore a new era in medical microbiology is demanding a rapid transition from current procedures to high throughput analytical systems for the diagnosis of microbial pathogens. This book covers the broad microbiological applications of proteomics and mass spectrometry. It is divided into six sections that follow the general progression in which most microbiology laboratories are approaching the subject -Transition, Tools, Preparation, Profiling by Patterns, Target Proteins, and Data Analysis.

InhaltsangabePreface List of contributors Microbial Characterisation; the Transition from Conventional Methods to Proteomics. 1) CHANGING CONCEPTS IN THE CHARACTERISATION OF MICROBES AND THE INFLUENCE OF MASS SPECTROMETRY Haroun Shah et al 2) MICROBIAL PHYLOGENY AND EVOLUTION BASED ON PROTEIN SEQUENCES (THE CHANGE FROM TARGETED GENES TO PROTEINS) Radhey Gupta 2: PROTEOMICS TOOLS AND BIOMARKER DISCOVERY. 3) OVERVIEW OF THE PROTEOMIC TOOLS AND IT LINKS TO GENOMICS Raju Misra. 4) HIGH THROUGHPUT BIOMARKER DISCOVERY IN MICROORGANISMS Ming Fang Proteomic Strategies for Protein Identification 1. Bottomup Proteomics 2. Topdown Proteomics Multidimensional Protein Identification Mass Spectrometry Based Targeted Protein Quantification and Biomarker Discovery Selected Reaction Monitoring Conclusions 5) MALDI MASS SPECTROMETRY IMAGING, A NEW FRONTIER IN BIOSTRUCTURAL TECHNIQUES: APPLICATIONS IN BIOMEDICINE Simona Francese and Malcolm R. Clench 3: PROTEIN SAMPLES PREPARATION TECHNIQUES CONVENTIONAL APPROACHES FOR SAMPLE PREPARATION FOR LIQUID CHROMATOGRAPHY AND TWO-DIMENSIONAL GEL ELECTROPHORESIS Vesela Encheva and Robert Parker 7) ISOLATION AND PREPARATION OF SPORE PROTEINS AND SUBSEQUENT CHARACTERISATION BY ELECTROPHORESIS AND MASS SPECTROMETRY Nicola Thorne, Saheer Gharbia and Haroun Shah 8) CHARACTERIZATION OF BACTERIAL MEMBRANE PROTEINS USING A NOVEL COMBINATION OF A LIPID BASED PROTEIN IMMOBILIZATION TECHNIQUE WITH MASS SPECTROMETRY Roger Karlsson, Darren Chooneea, Elisabet Carlsohn, Vesela Encheva and Haroun Shah 9) Wider Protein Detection from Biological Extracts by the Reduction of Dynamic Concentration Range. Luc Guerrier, Egisto Boschetti and Piergiorgi Roghetti 10) 3Dgel electrophoresis a new development in protein analysis. Robert Ventzki and Josef Stegemann SECTION 4: CHARACTERISATION OF MICROORGANISMS BY PATTERN MATCHING OF MASS SPECTRAL PROFILES AND BIOMARKER APPROACHES REQUIRING MINIMAL SAMPLE PREPARATION. 11) Microbial Disease Biomarkers using ProteinChip Arrays Shea Hamilton, Michael Levin, J. Simon Kroll, Paul R. Langford 12) MALDITOF MS and microbial identification: years of experimental development to an established protocol. Wibke Kallow, Marcel Erhard, Haroun N. Shah, Emmanuel Raptakis, Martin Welker. 5: Targeted Molecules and Analysis of Specific Microorganisms. 13) Whole Cell MALDI Mass Spectrometry for the Rapid Characterisation of Bacteria; A Survey of Applications to Major Phyletic Lines in Microbial Kingdom. Ben van Baar Bacillus spp. Staphylococcus spp. Streptococcus spp. Mycobacterium spp. Other Grampositive bacteria Escherichia coli Gramnegative food and waterborne pathogen proteobacteria, other than E. Coli Typical sexually transmitted pathogens: Neisseria spp. and Haemophilus spp. Gramnegative biothreat agent bacteria Other Gramnegative bacteria Pathogenic Cyanobacteria Strategies for the identification of biomarkers in whole cell MALDI MS spectra Protein database consideration Ontarget treatment and analysis Offtarget' Analysis and correlation with proteomics studies General consideration of biomarker identification strategies Conclusions and outlook 14) The power of Gel-based proteomics to understand physiology in Bacillus subtilis Haike Antelmann and Michael Hecker Introduction Results 1 Proteomics of protein secretion mechanisms in Bacillus subtilis 2 Definition of proteomic signatures to study cell physiology 3 Proteomics as tool to visualize reversible and irreversible thiol- modifications 4 Proteomics as tool to define regulon structures and targets for non- coding RNAs 5 Acknowledgment 15) Mass Spectrometry in the study of Tularemia Pathogenesis. Jiri Stulik, Juraj Lenco, Jiri Dresler, Jana Klimentova, Lenka Hernychova, Lucie Balonova and Alena Fucikova. References 16) Bacterial Post-G

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