The first protocols book, Free Radical and Antioxidant Protocols (1) was published in late 1998. Sections were divided into three parts, covering selected biochemical techniques for measuring oxidative stress, antioxidant (AOX) activity, and combined applications. In choosing the 40 methods to be included in that book, I realized there were considerably more of equal value than that which we could have presented in a single volume. To produce a comprehensive resource, this book and a third are being compiled to expand coverage of the field. A summary of papers (2) published on this important subject emphasizes the continuing rapid growth in oxidative stress investigations relating to our understanding of biochemical reactions, their relevance to pathophysiological mechanisms, how disease may arise, and how therapeutic intervention may be achieved(3). Although there is some overlap between the categories, the ana- sis shown below illustrates where current studies are concentrated and are almost evenly distributed between free radicals and AOX. Over the last 4 yr, there has been a 55% increase in the number of papers published in the area.

InhaltsangabePart I. Techniques for Free Radical Derived Biomarkers. Human Xanthine Oxidoreductase Determination by a Competitive ELISA Maria Giulia Battelli and Silvia Musiani Simultaneous Determination of Polyunsaturated Fatty Acids and Corresponding Monohydroperoxy and Monohydroxy Peroxidation Products by HPLC Richard W. Browne and Donald Armstrong Determination of Products of Lipid Oxidation by Infrared Spectroscopy Douglas Borchman and Santosh Sinha Detection of Docosahexaenoic Acid Hydroperoxides in Retina by Gas Chromatography/Mass Spectrometry GueyShuang Wu and Narsing A. Rao Detection of Lipid Hydroperoxide-Derived Protein Modification with Polyclonal Antibodies Yoji Kato and Toshihiko Osawa Techniques for Determining the Metabolic Pathways of Eicosanoids and for Evaluating the Rate-Controlling Enzymes Ninder Panesar, Yashwant G. Deshpande, and Donald L. Kaminski Mass Spectrometric Quantification of F2-Isoprostanes as Indicators of Oxidative Stress Jason D. Morrow and L. Jackson Roberts, II Formation of Apolipoprotein AI-AII Heterodimers by Oxidation of High-Density Lipoprotein Audric S. Moses and Gordon A. Francis Detection of Certain Peroxynitrite-Induced DNA Modifications Hiroshi Ohshima, László Virág, Jose Souza, Vladimir Yermilov, Brigitte Pignatelli, Mitsuharu Masuda, and Csaba Szabó Hydroxyl and 1-Hydroxyethyl Radical Detection by Spin Trapping and GC-MS José A. Castro and Gerardo D. Castro Analysis of Aliphatic Amino Acid Alcohols in Oxidized Proteins Bénédicte Morin, Shanlin Fu, Hongjie Wang, Michael J. Davies, and Roger T. Dean Rapid Determination of Glutamate Using HPLC Technology Aqeela Afzal, Mohammed Afzal, Andrew Jones, and Donald Armstrong A Rapid Method for the Quantification of GSH and GSSG in Biological Samples Mohammed Afzal, AqeelaAfzal, Andrew Jones, and Donald Armstrong Protein Carbonyl Measurement by ELISA I. Hendrikje Buss and Christine C. Winterbourn Ne(carboxymethyl)lysine (CML) as a Biomarker of Oxidative Stress in LongLived Tissue Proteins J. Nikki Shaw, John W. Baynes, and Suzanne R. Thorpe Measurement of S-Glutathionated Hemoglobin in Human Erythrocytes by Isoelectric Focusing Electrophoresis HawWen Chen and ChongKuei Lii Oxidation of Cellular DNA Measured with the Comet Assay Andrew R. Collins and Mária Duþs inská Measurement of DNA Double-Strand Breaks with Giant DNA and High Molecular-Weight DNA Fragments by Pulsed-Field Gel Electrophoresis Yoshihiro Higuchi Evaluation of Antibodies Against Oxygen Free Radical-Modified DNA by ELISA Rashid Ali and Khurshid Alam Part II. Techniques for Antioxidant Biomarkers Simultaneous Analysis of Multiple Redox-Active Metabolites from Biological Matrices Bruce S. Kristal, Karen Vigneau-Callahan, and Wayne R. Matson Determination of Uric Acid in Urine by Fast-Scan Voltammetry (FSV) Using a Highly Activated Carbon Fiber Electrode Roberto Bravo, Dawn M. Stickle, and Anna Brajter-Toth Measurement of a-Tocopherol Turnover in Plasma and in Lipoproteins Using Stable Isotopes and Gas Chromatography/Mass Spectrometry Elizabeth J. Parks Analysis of Tocotrienols in Different Sample Matrixes by HPLC Kalyana Sundram and Rosnah Md. Nor Measurement of b-Carotene15,15'-Dioxygenase Activity by Reverse-Phase HPLC Alexandrine During, Akihiko Nagao, and James Cecil Smith, Jr. Ubiquinol/Ubiquinone Ratio as a Marker of Oxidative Stress Yorihiro Yamamoto and Satoshi Yamashita Catechol and PyrogallolType Flavonoids: Analysis of Tea Catechins in Plasma Keizo Umegaki, Mituaki Sano, and Isoa Tomita Pyruvate Dehydrogenase Complex as a

InhaltsangabePart I. Techniques for Free Radical Derived Biomarkers. Human Xanthine Oxidoreductase Determination by a Competitive ELISA Maria Giulia Battelli and Silvia Musiani Simultaneous Determination of Polyunsaturated Fatty Acids and Corresponding Monohydroperoxy and Monohydroxy Peroxidation Products by HPLC Richard W. Browne and Donald Armstrong Determination of Products of Lipid Oxidation by Infrared Spectroscopy Douglas Borchman and Santosh Sinha Detection of Docosahexaenoic Acid Hydroperoxides in Retina by Gas Chromatography/Mass Spectrometry GueyShuang Wu and Narsing A. Rao Detection of Lipid Hydroperoxide-Derived Protein Modification with Polyclonal Antibodies Yoji Kato and Toshihiko Osawa Techniques for Determining the Metabolic Pathways of Eicosanoids and for Evaluating the Rate-Controlling Enzymes Ninder Panesar, Yashwant G. Deshpande, and Donald L. Kaminski Mass Spectrometric Quantification of F2-Isoprostanes as Indicators of Oxidative Stress Jason D. Morrow and L. Jackson Roberts, II Formation of Apolipoprotein AI-AII Heterodimers by Oxidation of High-Density Lipoprotein Audric S. Moses and Gordon A. Francis Detection of Certain Peroxynitrite-Induced DNA Modifications Hiroshi Ohshima, László Virág, Jose Souza, Vladimir Yermilov, Brigitte Pignatelli, Mitsuharu Masuda, and Csaba Szabó Hydroxyl and 1-Hydroxyethyl Radical Detection by Spin Trapping and GC-MS José A. Castro and Gerardo D. Castro Analysis of Aliphatic Amino Acid Alcohols in Oxidized Proteins Bénédicte Morin, Shanlin Fu, Hongjie Wang, Michael J. Davies, and Roger T. Dean Rapid Determination of Glutamate Using HPLC Technology Aqeela Afzal, Mohammed Afzal, Andrew Jones, and Donald Armstrong A Rapid Method for the Quantification of GSH and GSSG in Biological Samples Mohammed Afzal, Aqeela Afzal, Andrew Jones, and Donald Armstrong Protein Carbonyl Measurement by ELISA I. Hendrikje Buss and Christine C. Winterbourn Ne(carboxymethyl)lysine (CML) as a Biomarker of Oxidative Stress in LongLived Tissue Proteins J. Nikki Shaw, John W. Baynes, and Suzanne R. Thorpe Measurement of S-Glutathionated Hemoglobin in Human Erythrocytes by Isoelectric Focusing Electrophoresis HawWen Chen and ChongKuei Lii Oxidation of Cellular DNA Measured with the Comet Assay Andrew R. Collins and Mária Duþs inská Measurement of DNA Double-Strand Breaks with Giant DNA and High Molecular-Weight DNA Fragments by Pulsed-Field Gel Electrophoresis Yoshihiro Higuchi Evaluation of Antibodies Against Oxygen Free Radical-Modified DNA by ELISA Rashid Ali and Khurshid Alam Part II. Techniques for Antioxidant Biomarkers Simultaneous Analysis of Multiple Redox-Active Metabolites from Biological Matrices Bruce S. Kristal, Karen Vigneau-Callahan, and Wayne R. Matson Determination of Uric Acid in Urine by Fast-Scan Voltammetry (FSV) Using a Highly Activated Carbon Fiber Electrode Roberto Bravo, Dawn M. Stickle, and Anna Brajter-Toth Measurement of a-Tocopherol Turnover in Plasma and in Lipoproteins Using Stable Isotopes and Gas Chromatography/Mass Spectrometry Elizabeth J. Parks Analysis of Tocotrienols in Different Sample Matrixes by HPLC Kalyana Sundram and Rosnah Md. Nor Measurement of b-Carotene15,15'-Dioxygenase Activity by Reverse-Phase HPLC Alexandrine During, Akihiko Nagao, and James Cecil Smith, Jr. Ubiquinol/Ubiquinone Ratio as a Marker of Oxidative Stress Yorihiro Yamamoto and Satoshi Yamashita Catechol and PyrogallolType Flavonoids: Analysis of Tea Catechins in Plasma Keizo Umegaki, Mituaki Sano, and Isoa Tomita Pyruvate Dehydrogenase Complex as a Marker of Mitochondrial Metabolism: Inhibition by 4-Hydroxy-2-Nonenal, Mulchand S. Patel and Lioubov G. Korotchkina Ceruloplasmin Detection by SDS-PAGE, Immunoblotting, and Leseprobe

Part I. Techniques for Free Radical Derived Biomarkers. Human Xanthine Oxidoreductase Determination by a Competitive ELISA Maria Giulia Battelli and Silvia Musiani Simultaneous Determination of Polyunsaturated Fatty Acids and Corresponding Monohydroperoxy and Monohydroxy Peroxidation Products by HPLC Richard W. Browne and Donald Armstrong Determination of Products of Lipid Oxidation by Infrared Spectroscopy Douglas Borchman and Santosh Sinha Detection of Docosahexaenoic Acid Hydroperoxides in Retina by Gas Chromatography/Mass Spectrometry Guey-Shuang Wu and Narsing A. Rao Detection of Lipid Hydroperoxide-Derived Protein Modification with Polyclonal Antibodies Yoji Kato and Toshihiko Osawa Techniques for Determining the Metabolic Pathways of Eicosanoids and for Evaluating the Rate-Controlling Enzymes Ninder Panesar, Yashwant G. Deshpande, and Donald L. Kaminski Mass Spectrometric Quantification of F2-Isoprostanes as Indicators of Oxidative Stress Jason D. Morrow and L. Jackson Roberts, II Formation of Apolipoprotein AI-AII Heterodimers by Oxidation of High-Density Lipoprotein Audric S. Moses and Gordon A. Francis Detection of Certain Peroxynitrite-Induced DNA Modifications Hiroshi Ohshima, Laszlo Virag, Jose Souza, Vladimir Yermilov, Brigitte Pignatelli, Mitsuharu Masuda, and Csaba Szabo Hydroxyl and 1-Hydroxyethyl Radical Detection by Spin Trapping and GC-MS Jose A. Castro and Gerardo D. Castro Analysis of Aliphatic Amino Acid Alcohols in Oxidized Proteins Benedicte Morin, Shanlin Fu, Hongjie Wang, Michael J. Davies, and Roger T. Dean Rapid Determination of Glutamate Using HPLC Technology Aqeela Afzal, Mohammed Afzal, Andrew Jones, and Donald Armstrong A Rapid Method for the Quantification of GSH and GSSG in Biological Samples Mohammed Afzal, Aqeela Afzal, Andrew Jones, and Donald Armstrong Protein Carbonyl Measurement by ELISA I. Hendrikje Buss and Christine C. Winterbourn Ne-(carboxymethyl)lysine (CML) as a Biomarker of Oxidative Stress in Long-Lived Tissue Proteins J. Nikki Shaw, John W. Baynes, and Suzanne R. Thorpe Measurement of S-Glutathionated Hemoglobin in Human Erythrocytes by Isoelectric Focusing Electrophoresis Haw-Wen Chen and Chong-Kuei Lii Oxidation of Cellular DNA Measured with the Comet Assay Andrew R. Collins and Maria Duþs inska Measurement of DNA Double-Strand Breaks with Giant DNA and High Molecular-Weight DNA Fragments by Pulsed-Field Gel Electrophoresis Yoshihiro Higuchi Evaluation of Antibodies Against Oxygen Free Radical-Modified DNA by ELISA Rashid Ali and Khurshid Alam Part II. Techniques for Antioxidant Biomarkers Simultaneous Analysis of Multiple Redox-Active Metabolites from Biological Matrices Bruce S. Kristal, Karen Vigneau-Callahan, and Wayne R. Matson Determination of Uric Acid in Urine by Fast-Scan Voltammetry (FSV) Using a Highly Activated Carbon Fiber Electrode Roberto Bravo, Dawn M. Stickle, and Anna Brajter-Toth Measurement of a-Tocopherol Turnover in Plasma and in Lipoproteins Using Stable Isotopes and Gas Chromatography/Mass Spectrometry Elizabeth J. Parks Analysis of Tocotrienols in Different Sample Matrixes by HPLC Kalyana Sundram and Rosnah Md. Nor Measurement of b-Carotene15,15'-Dioxygenase Activity by Reverse-Phase HPLC Alexandrine During, Akihiko Nagao, and James Cecil Smith, Jr. Ubiquinol/Ubiquinone Ratio as a Marker of Oxidative Stress Yorihiro Yamamoto and Satoshi Yamashita Catechol- and Pyrog ...