This new edition of Natural Products Isolation has been not only significantly updated to reflect recent advances in the field, but has also been expanded with three new chapters on hyphenated techniques, purification by solvent extraction using partition coefficient, and isolation of microbial natural products. The book includes powerful cutting-edge techniques and simple basic techniques that can save time and effort, as well as achieve results equivalent to using some of the more modern and expensive methodologies. The protocols follow the successful Methods in Molecular Biologya''¢ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Natural Product Isolation: An Overview Satyajit D. Sarker, Zahid Latif, and Alexander I. Gray Initial and Bulk Extraction Veronique Seidel Supercritical Fluid Extraction Lutfun Nahar and Satyajit D. Sarker An Introduction to Planar Chromatography Simon Gibbons Isolation of Natural Products by Low-Pressure Column Chromatography Raymond G. Reid and Satyajit D. Sarker Isolation by Ion-Exchange Methods David G. Durham Separation by High-Speed Countercurrent Chromatography James B. McAlpine and Patrick Morris Isolation by Preparative High-Performance Liquid Chromatography Zahid Latif Hyphenated Techniques Satyajit D. Sarker and Lutfun Nahar Purification by Solvent Extraction Using Partition Coefficient Hideaki Otsuka Crystallization in Final Stages of Purification Alastair J. Florence, Norman Shankland, and Andrea Johnston Dereplication and Partial Identification of Compounds Laurence Dinan Extraction of Plant Secondary Metabolites William P. Jones and A. Douglas Kinghorn Isolation of Marine Natural Products Wael E. Houssen and Marcel Jaspars Isolation of Microbial Natural Products Russell A. Barrow Purification of Water-Soluble Natural Products Yuzuru Shimizu and Bo Li Scale-Up of Natural Product Isolation Steven M. Martin, David A. Kau, and Stephen K. Wrigley Follow-Up of Natural Product Isolation Richard J. P. Cannell Index

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