The evaluation of potential mutagenic activity is a critical step in the assessment of the safety of both new and pre-existing chemical types. In Genetic Toxicology: Principles and Methods, expert contributors help to satisfy the demand for education in this tremendously important area of study.  The volume covers three basic areas: the scientific basis of the discipline, the methodologies of the main test assays, and the application of the methods, all aimed primarily at scientists in the safety departments of the industries working with both natural and synthetic chemicals.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Intuitive and cutting-edge, Genetic Toxicology: Principles and Methods provides crucial support to both laboratory workers in providing quality information on the appropriate application of techniques and to study directors in their assay selection and protocol design in this vital field.

Inhaltsangabe1. The Application of Structure Activity Relationships to the Prediction of the Mutagenic Activity of Chemicals            Philip Judson 2. Bacterial Mutagenicity Assays: Test Methods            David Gatehouse 3. The Mouse Lymphoma Assay            Melvyn Lloyd and Darren Kidd 4. Mammalian Cell HPRT Gene Mutation Assay: Test Methods            George E. Johnson 5. The In Vitro Mammalian Chromosome Aberration Test            Gill Clare 6. The Interpretation and Analysis of Cytogenetic Data            Natalie Danford 7. The In Vitro Micronucleus Assay            Ann T. Doherty 8. The In Vitro and In Vivo Comet Assays            Brian Burlinson 9. Assessment of DNA Interstrand Crosslinks (ICLs) Using the Modified Alkaline Comet Assay            Jian Hong Wu and Nigel J. Jones 10. 32P-Postlabelling for the Sensitive Detection of DNA Adducts            Nigel J. Jones 11. Methods for the Detection of DNA Adducts            Karen Brown 12. The GADD45a-GFP GreenScreen HC Assay            Richard M. Walmsley and Matthew Tate 13. Real Time Reverse-Transcription Polymerase Chain Reaction (RT-PCR): Technical Considerations for Gene Expression Analysis            Shareen H. Doak and Zoulikha M. Zaïr 14. Cytogenetic In Vivo Assays in Somatic Cells            Ann T. Doherty, Adolph Baumgartner, and Diana Anderson 15. Cytogenetic Methods in Human Biomonitoring: Principles and Uses            Raluca A. Mateuca, Ilsa Decordier, and Micheline Kirsch-Volders 16. The Measurement of Induced Genetic Change in Mammalian Germ Cells            Ilse-Dore Adler, Francesca Pacchierotti, and Antonella Russo 17. Transgenic Animal Mutation Models: A Review of the Models and How They Function            Steve Dean 18. Analysis of Genotoxicity Data in a Regulatory Context            Ian de G. Mitchell and David O.F. Skibinski

1. The Application of Structure Activity Relationships to the Prediction of the Mutagenic Activity of Chemicals             Philip Judson   2. Bacterial Mutagenicity Assays: Test Methods             David Gatehouse   3. The Mouse Lymphoma Assay             Melvyn Lloyd and Darren Kidd   4. Mammalian Cell HPRT Gene Mutation Assay: Test Methods             George E. Johnson   5. The In Vitro Mammalian Chromosome Aberration Test             Gill Clare   6. The Interpretation and Analysis of Cytogenetic Data             Natalie Danford   7. The In Vitro Micronucleus Assay             Ann T. Doherty   8. The In Vitro and In Vivo Comet Assays             Brian Burlinson   9. Assessment of DNA Interstrand Crosslinks (ICLs) Using the Modified Alkaline Comet Assay             Jian Hong Wu and Nigel J. Jones   10. 32P-Postlabelling for the Sensitive Detection of DNA Adducts             Nigel J. Jones   11. Methods for the Detection of DNA Adducts             Karen Brown   12. The GADD45a-GFP GreenScreen HC Assay             Richard M. Walmsley and Matthew Tate   13. Real Time Reverse-Transcription Polymerase Chain Reaction (RT-PCR): Technical Considerations for Gene Expression Analysis             Shareen H. Doak and Zoulikha M. Zaïr   14. Cytogenetic In Vivo Assays in Somatic Cells             Ann T. Doherty, Adolph Baumgartner, and Diana Anderson   15. Cytogenetic Methods in Human Biomonitoring: Principles and Uses             Raluca A. Mateuca, Ilsa Decordier, and Micheline Kirsch-Volders   16. The Measurement of Induced Genetic Change in Mammalian Germ Cells             Ilse-Dore Adler, Francesca Pacchierotti, and Antonella Russo   17. Transgenic Animal Mutation Models: A Review of the Models and How They Function             Steve Dean   18. Analysis of Genotoxicity Data in a Regulatory Context             Ian de G. Mitchell and David O.F. Skibinski