The innate immune response is a crucial component of early resistance to infection, and it is now revealing increasing levels of complexity. The ability to modify the genome in vivo, has facilitated understanding of complex interactions between leucocytes and other components of the immune system, and phenotype-driven strategies using chemical mutagenesis have placed another powerful weapon in the armamentarium. In, Leucocytes: Methods and Protocols provides detailed protocols and practical advice on a variety of modern approaches to the study of leucocytes and their products.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Leucocytes: Methods and Protocols seeks to aid scientists in further study into the immune system and leucocytes.

Table of Contents Chapter Title Author 1 ENU Based Phenotype Driven Screening   Vera  M. Ripoll, Philip L. Kong, and Paul K. Potter 2 Detection and Quantification of Cytokines and Other Biomarkers   Evan L. Chiswick, Elizabeth Duffy, Brian Japp and Daniel Remick 3 FLOW CYTOMETRY ANALYSIS OF CELL CYCLING AND PROLIFERATION IN MOUSE HEMATOPOIETIC STEM AND PROGENITOR CELLS   Valerie Barbier, Bianca Nowlan, Jean-Pierre Levesque and Ingrid G. Winkler 4 Flow Cytometry Measurement of Bone Marrow Perfusion in the Mouse and Sorting of Progenitors and Stems Cells According to Position Relative to Blood Flow in Vivo   Valerie Barbier, Ingrid G. Winkler, Robert Wadley and Jean-Pierre Levesque 5 Analyzing Cell Death Events in Cultured Leukocytes Karin Christenson, Fredrik B. Thoren and Johan Bylund 6 Towards a four-dimensional view of neutrophils   Ben A. Croker, Andrew W. Roberts and Nicos A. Nicola 7 Isolation of Human and Mouse Neutrophils Ex vivo and In vitro Yan Hu   8 MEASUREMENT OF OXIDATIVE BURST IN NEUTROPHILS   Yu Chen and Wolfgang G. Junger   9 Measurement of Neutrophil Elastase, Proteinase 3 and Cathepsin G Activities using Intramolecularly Quenched Fluorogenic Substrates   Brice Korkmaz, Sylvie Attucci, Christophe Epinette, Elodie Pitois, Marie-Lise Jourdan, Luiz Juliano and Francis Gauthier 10 The Macrophage C.P. Verschoor, A. Puchta and D.M.E. Bowdish 11 Generation and Characterization of MacGreen Mice, the Cfs1r-EGFP Transgenic Mice R. Tedjo Sasmono and Elizabeth Williams    12 Generation of Mouse Bone Marrow-derived Macrophages   Silvia Manzanero   13 Isolation and Differentiation of Monocytes-macrophages from Human Blood   Dipti Vijayan   14 In Vitro Measurement of Phagocytosis and Killing of Cryptococcus neoformans by Macrophages Andre Moraes Nicola and Arturo Casadevall   15 Measuring the Inflammasome Olaf Groß 16   Arginine and Macrophage Activation   Mònica Comalada, Andree Yeramian, Manuel Modolell, Jorge Lloberas and Antonio Celada 17 Immunodetection of Granzyme B Tissue Distribution and Cellular Localisation   Catherina H. Bird,Corrine Hitchen , Mark Prescott, Ian Harper and Phillip I. Bird 18 Detection of Human and Mouse Granzyme B Activity in Cell Extracts   Sarah Elizabeth Stewart, Matthew Stephen James Mangan, Phillip Ian Bird and Dion Kaiserman 19 T cell Transfer Model of Colitis: A Great Tool to Assess the Contribution of T cells in Chronic Intestinal Inflammation   Rajaraman Eri, Michael A. McGuckin and Robert Wadley   20 Measurement of Nitrite in Urine by Gas Chromatography-Mass Spectrometry Dimitrios Tsikas, Maria-Theresia Suchy, Anja , Bibiana Beckmann and Frank-Mathias Gutzki

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