InhaltsangabePart I. In silico Methods 1. Use of IMGT® Databases and Tools for Antibody Engineering and HumanizationMarie-Paule Lefranc, François Ehrenmann, Chantal Ginestoux, Véronique Giudicelli, and Patrice Duroux 2. Computer-Assisted Modeling of Antibody Variable DomainsOscar H. P. Ramos  Part II. Generation of Diversity 3. Cloning Single-Chain Antibody Fragments (scFv) from Hybridoma CellsLars Toleikis and André Frenzel 4. Human in Cell scFv Library from Infiltrating B CellSylvie Peraldi-Roux                                    5. Construction of Human Naive Antibody Gene LibrariesMichael Hust, André Frenzel, Torsten Meyer, Thomas Schirrmann, and Stefan Dübel    6. Synthetic Customized scFv Libraries  Gautier Robin and Pierre Martineau         7. Selection of Stable scFv Antibodies by Phage Display       Eeva-Christine Brockmann        8. Generation of Single Domain Antibody Fragments Derived from Camelids and Generation of Manifold Constructs Cécile Vincke, Carlos Gutiérez, Ulrich Wernery, Nick Devoogdt, Gholamreza Hassanzadeh-Ghassabeh, and Serge Muyldermans 9. Generation and Isolation of Target Specific Single Domain Antibodies from Shark Immune Repertoires Mischa Roland Müller, Ronan O'Dwyer, Marina Kovaleva, Fiona Rudkin, Helen Dooley, and Caroline Jane Barelle 10. Generation of Human Single Domain Antibody Repertoires by Kunkel Mutagenesis Kip Dudgeon, Romain Rouet, Kristoffer Famm, and Daniel Christ  Part IV. Selections of Lead Candidate 11. Phage Display and Selections on Purified AntigensJulie Matz and Patrick Chames     12. Phage Display and Selections on Cells Klervi Even-Desrumeaux and Patrick Chames  13. Humanization by CDR Grafting and Specificity-Determining Residue Grafting  Jin Hong Kim and Hyo Jeong Hong 14. Humanization by Guided Selections Sang Jick Kim and Hyo Jeong Hong             15. Selection of Antibody Fragments by Yeast Display   Nathalie Scholler       16. Evolution of Antibodies in vitro by Ribosome DisplayBryan M. Edwards and Mingyue He               17. Mammalian Cell Display of Full Length IgG        Chen Zhou and Wenyan (David) Shen Part V. Production of Recombinant Antibodies 18. Production of Antibody Fragments in Escherichia coli Tomohisa Katsuda, Hiroyuki Sonoda, and Hideki Yamaji         19. Production of Antibody Derivatives in the Methylotrophic Yeast Pichia pastorisSteve Schoonooghe, Jannick Leoen, and Jurgen Haustraete                  20. Monoclonal Antibody Expression in Mammalian Cells Richard (Yi) Zhang and Wenyan (David) Shen         21. Production of Recombinant Antibodies in Drosophila melanogaster S2 Cells Daniel X. Johansson, Thomas Krey, and Oskar Andersson 22. Production of Antibody Fragments Using the Baculovirus-Insect Cell System Takanori Furuta, Takafumi Ogawa, and Hideki Yamaji                  23. Transient and Stable Expression of Antibodies in Nicotiana SpeciesJ. Christopher Hall, Michael D. McLean and Freydoun Garabagi     Part VI. Variable Domain Optimization 24. Measuring Antibody-Antigen Binding Kinetics Using Surface Plasmon ResonanceStephen Hearty, Paul Leonard, and Richard O'Kennedy                25. Affinity Determination of Biotinylated Antibodies by Flow CytometryKlervi Even-Desrumeaux and Patrick Chames        26. Affinity Maturation of Antibodies: Optimized Methods to Generate High Quality scFv Libraries and Isolate IgG Candidates by High Throughput ScreeningLau

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