This study includes on the productivity of L-asparaginase by six different isolates of Acinetobacter baumannii and cell immobilization to produce L-asparaginase enzyme S4 isolated from sputum samples gave the highest production level of enzyme. The maximum activity ( 46.70 U/ml) using M9 medium supplemented with 0.5% soluble starch as carbon source and 0.5% L-asparagine as nitrogen source, Cell immobilization was recorded by physical immobilization and sawdust was the most favorable for the production of the highest L-asparaginase activity 41.2 U/ ml. Entrapment immobilization was the most suitable with sodium alginate.

Dr.Sahira Nsayif Muslim Assistant professor in Microbiology .coll. Sci. ,Al-Mustansiriya univ. -,M.Sci. and Ph.D in Microbiology .Dr.Shatha Ali Shafiq Assistant professor in Mycotoxicology ,coll. Sci. ,Al-Mustansiriya univ. Member at the Association of University lecturers and member in Iraqi Biological Organization -Baghdad-Iraq.