This book presents a compendium of methodologies for the study of membrane lipids, varying from traditional lab bench experimentation to computer simulation and theoretical models. The volume provides a comprehensive set of techniques for studying membrane lipids with a strong biophysical emphasis. It compares the various available techniques including the pros and cons as seen by the experts.

Methods in Membrane Lipids: Table of Contents Introductory Chapters: 1 A glance at the structural and functional diversity of membrane lipids Alejandro M. Dopico and Gabor J. Tigyi 2 Membrane lipid polymorphism: relationship to bilayer properties and protein function Richard Epand Lipid distribution, membrane lipid structure, and lipid-lipid interactions: 3 Acrylodan-labeled intestinal fatty acid binding protein to measure concentrations of unbound fatty acids Jeffrey R. Simard, Frits Kamp, and James A. Hamilton 4 Measuring molecular order and orientation using Coherent-anti Stokes Raman scattering microscopy Hilde A. Rinia, George W.H. Wurpel, and Michiel Müller 5 Preparation of oriented, fully hydrated lipid samples for structure determination using X-ray scattering Stephanie Tristram-Nagle 6 Nuclear Magnetic Resonance investigation of oriented lipid membranes Olivier Soubias and Klaus Gawrisch 7 Molecular dynamics simulations as a complement to Nuclear Magnetic Resonance and X-ray diffraction measurements Scott Feller 8 Use of inverse theory algorithms in the analysis of biomembrane Nuclear Magnetic Resonance data Edward Sternin 9 Statistical thermodynamics via computer simulation to characterize phospholipid interactions in membranes Mihaly Mezei and Pal Jedlovszky 10 Fluorometric assay for detection of sterol oxidation in liposomal membranes Parkson L-G. Chong and Michelle Olsher 11 Fluorescence detection of signs of sterol superlattice formation in lipid membranes Parkson L-G. Chong, Berenice Venegas, and Michelle Olsher Characterization of lipid phases: 12 Differential scanning calorimetry in the study of lipid phase transitions in model and biological membranes: practical considerations Ruthven N. A. H. Lewis, David A. Mannock, and Ronald N. McElhaney 13 Pressure-perturbation calorimetry Heiko Heerklotz 14 Fourier transform infrared spectroscopy in the study of lipid phase transitions in model and biological membranes: practical considerations Ruthven N. A. H.Lewis and Ronald McElhaney 15 Optical dynamometry to study phase transitions in lipid membranes Rumiana Dimova and Bernard Pouligny Lipid movements and diffusion: 16 Fluorescence assays for measuring fatty acid binding and transport through membranes Kellen Brunaldi, Jeffrey R. Simard, Frits Kamp, Charu Rewal, Tanong Asawakarn, Paul O'Shea, and James A. Hamilton 17 Measurement of lateral diffusion rates in membranes by Pulsed Magnetic Field Gradient, Magic Angle-Spinning Proton Nuclear Magnetic Resonance Klaus Gawrisch and Holly Gaede 18 Using Fluorescence Recovery After Photobleaching to measure diffusion in membranes Conrad W. Mullineaux and Helmut Kirchhoff 19 Single molecule fluorescence microscopy to determine phospholipid lateral diffusion Michael J. Murcia, Sumit Garg, and Christoph Naumann 20 Modeling two- and three-dimensional diffusion Michael Saxton 21 Measurement of water and solute permeability by stopped-flow fluorimetry John C. Mathai and Mark L. Zeidel Pressure between lipids, mono- and bilayer lipid curvature and stress: 22 Fluorescence microscopy to study pressure between lipids in giant unilamelar vesicles Anna Celli, Claudia Y.C. Lee, and Enrico Gratton 23 X-ray scattering and solid-state deuterium Nuclear Magnetic Resonance probes of structural fluctuations in lipid membranes Horia I. Petrache and Michael F. Brown 24 Determination of lipid spontaneous curvature from X-ray examinations of inverted hexagonal phases Michael Kozlov 25 Shape analysis of giant vesicles with fluid phase coexistence by laser scanning microscopy to determine curvature, bending elasticity and line tension Samuel T. He ...

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