The present write up gives a insight on Harvesting of Cells, Cell killing curve, Extraction of protein from eukaryotes, Estimation of protein, SDS-PAGE, 2DE, LC-MS, Mass Spectrometry, extraction and purification RNA, genomic DNA, RNA, Agarose gel electrophoresis of DNA and Extraction from Agarose Gel, DNA transformation, ELISA, Staining includes Monochrome, Negative, and Grams staining of microorganism

Dr. LH Kamble is associate professor in Biotechnology, SRTM University, Nanded MH India. He has masters in Biochemistry and Bioinformatics. Dr. SV Mulange has completed doctoral work on proteomic analysis, Masters in biophysics and expertise in proteomics and genomics.