ue Residue 15 5.1. Use of Filtration. 16 5.2. Centrifugation. 17 6. Removing Nonlipids from Lipid Extracts 6.1. Removal of Nonlipids by Evaporation and Reextraction. 17. 17 6.2. Prewashing the Tissue Sample. 6.3. Removal of Nonlipids by Precipitating the Lipids with the Proteins. 18. 19 6.4. Liquid/Liquid Partitioning. 19 6.5. Removal of Nonlipids by Dialysis 6.6. Separation from Nonlipids by Changing a 21 Partition Constant.

InhaltsangabeLipid Extraction.- Preparation and Analysis of Acyl and Alkenyl Groups of Glycerophospholipids from Brain Subcellular Membranes.- Quantitative Analysis of Acyl Group Composition of Brain Phospholipids, Neutral Lipids, and Free Fatty Acids.- Steroids and Related Isoprenoids.- Phospholipids.- Determination of Phospholipases, Lipases, and Lysophospholipases.- Isolation, Separation, and Analysis of Phosphoinositides from Biological Sources.- Analysis of Prostaglandins, Leukotrienes, and-Related Compounds in Retina and Brain.- HPLC Analysis of Neutral Glycosphingolipids and Sulfatides.- Methods to Study the Biochemistry of Gangliosides.