InhaltsangabePart I. Labeling DNA Breaks Using Terminal Transferase (TUNEL Assay) Labeling DNA Damage with Terminal Transferase: Applicability, Specificity, and Limitations P. Roy Walker, Christine Carson, Julie Leblanc, and Marianna Sikorska TUNEL Assay: An Overview of Techniques Deryk T. Loo Electron Microscopic Detection of DNA Damage Labeled by TUNEL Antonio Migheli Quantitative Differentiation of Both Free 3' OH and 5' OH DNA Ends Using Terminal Transferase-Based Labeling Combined with Transmission Electron Microscopy Yoshinori Otsuki and Yuko Ito Determination of Three-Dimensional Distribution of Apoptotic DNA Damage by Combination of TUNEL and Quick-Freezing and Deep-Etching Techniques Shinichi Ohno, Takeshi Baba, Nobuo Terada, and Yasuhisa Fujii In Situ Detection of DNA Strand Breaks in Analysis of Apoptosis by Flow- and Laser-Scanning Cytometry Zbigniew Darzynkiewicz, Elzbieta Bedner, and Piotr Smolewski Part II. Labeling DNA Breaks Using DNA Polymerase I or its Klenow Fragment DNA Damage Detection Using DNA Polymerase I or its Klenow Fragment: Applicability, Specificity, Limitations Jan Hein van Dierendonck Labeling DNA Breaks In Situ by Klenow Enzyme Katherine A. Wood In Situ Nick Translation at the Electron Microscopic Level Marc Thiry Part III. Labeling DNA Breaks Using Ligase In Situ DNA Ligation as a Method for Labeling Apoptotic Cells in Tissue Sections: An Overview Peter J. Hornsby and Vladimir V. Didenko Detection of Specific Double-Strand DNA Breaks and Apoptosis In Situ Using T4 DNA Ligase Vladimir V. Didenko In Situ Detection of Double-Strand DNA Breaks with Terminal 5'OH Groups Vladimir V. Didenko, Hop Ngo, and David S. Baskin Part IV. Detection of DNA Breaks in Agarose Trapped Cells: Comet Assay and Related Techniques The Comet Assay: Principles, Applications, and Limitations Andrew R. Collins The Comet Assay: An Overview of Techniques Peggy L. Olive Ultrasensitive Detection of DNA Damage by the Combination of the Comet and TUNEL Assays Andrei L. Kindzelskii and Howard R. Petty Application of FISH to Detect DNA Damage: DNA Breakage Detection-FISH (DBD-FISH) José Luis Fernández and Jaime Gosálvez Part V. Detection of Modified Bases and AP Sites in DNA Simultaneous In Situ Detection of DNA Fragmentation and RNA/DNA Oxidative Damage Using TUNEL Assay and Immunohistochemical Labeling for 8-Hydroxy-2'-Deoxyguanosine (8-OHdG) Alexander E. Kalyuzhny The In Situ Detection of Apurinic/Apyrimidinic Sites and DNA Breaks Bearing Extension Blocking Termini Philip K. Liu, Jiankun Cui, Niki Moore, and Dongya Huang Part VI. Indirect and General Markers of DNA Damage Markers of Poly (ADP-Ribose) Polymerase Activity as Correlates of DNA Damage Yinong Zhou, Shi Liang, and Lawrence R. Williams Ultrasound Imaging of Apoptosis: DNA-Damage Effects Visualized Gregory J. Czarnota, Michael C. Kolios, John W. Hunt, and Michael D. Sherar p53 Induction as an Indicator of DNA Damage Galina Selivanova Detection of Caspases Activation In Situ by Fluorochrome-Labeled Inhibitors of Caspases (FLICA) Zbigniew Darzynkiewicz, Elzbieta Bedner, Piotr Smolewski, Brian W. Lee, and Gary L. Johnson Index

Part I. Labeling DNA Breaks Using Terminal Transferase (TUNEL Assay) Labeling DNA Damage with Terminal Transferase: Applicability, Specificity, and Limitations P. Roy Walker, Christine Carson, Julie Leblanc, and Marianna Sikorska TUNEL Assay: An Overview of Techniques Deryk T. Loo Electron Microscopic Detection of DNA Damage Labeled by TUNEL Antonio Migheli Quantitative Differentiation of Both Free 3'' OH and 5'' OH DNA Ends Using Terminal Transferase-Based Labeling Combined with Transmission Electron Microscopy Yoshinori Otsuki and Yuko Ito Determination of Three-Dimensional Distribution of Apoptotic DNA Damage by Combination of TUNEL and Quick-Freezing and Deep-Etching Techniques Shinichi Ohno, Takeshi Baba, Nobuo Terada, and Yasuhisa Fujii In Situ Detection of DNA Strand Breaks in Analysis of Apoptosis by Flow- and Laser-Scanning Cytometry Zbigniew Darzynkiewicz, Elzbieta Bedner, and Piotr Smolewski Part II. Labeling DNA Breaks Using DNA Polymerase I or its Klenow Fragment DNA Damage Detection Using DNA Polymerase I or its Klenow Fragment: Applicability, Specificity, Limitations Jan Hein van Dierendonck Labeling DNA Breaks In Situ by Klenow Enzyme Katherine A. Wood In Situ Nick Translation at the Electron Microscopic Level Marc Thiry Part III. Labeling DNA Breaks Using Ligase In Situ DNA Ligation as a Method for Labeling Apoptotic Cells in Tissue Sections: An Overview Peter J. Hornsby and Vladimir V. Didenko Detection of Specific Double-Strand DNA Breaks and Apoptosis In Situ Using T4 DNA Ligase Vladimir V. Didenko In Situ Detection of Double-Strand DNA Breaks with Terminal 5''OH Groups Vladimir V. Didenko, Hop Ngo, and David S. Baskin Part IV. Detection of DNA Breaks in Agarose Trapped Cells: Comet Assay and Related Techniques The Comet Assay: Principles, Applications, and Limitations Andrew R. Collins The Comet Assay: An Overview of Techniques Peggy L. Olive Ultrasensitive Detection of DNA Damage by the Combination of the Comet and TUNEL Assays Andrei L. Kindzelskii and Howard R. Petty Application of FISH to Detect DNA Damage: DNA Breakage Detection-FISH (DBD-FISH) Jose Luis Fernandez and Jaime Gosalvez Part V. Detection of Modified Bases and AP Sites in DNA Simultaneous In Situ Detection of DNA Fragmentation and RNA/DNA Oxidative Damage Using TUNEL Assay and Immunohistochemical Labeling for 8-Hydroxy-2''-Deoxyguanosine (8-OHdG) Alexander E. Kalyuzhny The In Situ Detection of Apurinic/Apyrimidinic Sites and DNA Breaks Bearing Extension Blocking Termini Philip K. Liu, Jiankun Cui, Niki Moore, and Dongya Huang Part VI. Indirect and General Markers of DNA Damage Markers of Poly (ADP-Ribose) Polymerase Activity as Correlates of DNA Damage Yinong Zhou, Shi Liang, and Lawrence R. Williams Ultrasound Imaging of Apoptosis: DNA-Damage Effects Visualized Gregory J. Czarnota, Michael C. Kolios, John W. Hunt, and Michael D. Sherar p53 Induction as an Indicator of DNA Damage Galina Selivanova Detection of Caspases Activation In Situ by Fluorochrome-Labeled Inhibitors of Caspases (FLICA) Zbigniew Darzynkiewicz, Elzbieta Bedner, Piotr Smolewski, Brian W. Lee, and Gary L. Johnson Index