RNA interference has become a key method in the suppression of gene expression and the development of therapeutic agents, yet there is still the problem of delivery, stability, and the danger of off-target effects such as the silencing of unwanted genes and activation of innate immunity. In siRNA and miRNA Gene Silencing: From Bench to Bedside, expert researchers explore the most recent advances in siRNA design, expression, delivery, in vivo imaging, and methods to minimize siRNA's unwanted effects and promote successful use in patients. As part of the highly successful Methods in Molecular Biology series, the chapters focus on their respective subjects with easy-to-use, up-to-date information, including several step-by-step laboratory protocols on topics such as new delivery formulations and strategies with promising applications in vitro and in vivo, validated therapeutic target genes, and components of miRNA function, biogenesis, and interference with virus infection. Comprehensive and cutting-edge, siRNA and miRNA Gene Silencing: From Bench to Bedside offers an excellent collection of chapters to aid all those with an interest in RNAi, gene regulation, and new therapies.

Methods for Selecting Effective siRNA Sequences by using Statistical and Clustering Techniques Shigeru Takasaki Deciphering the Code of Innate Immunity Recognition of siRNAs Mouldy Sioud Peptide Targeted Delivery of Antisense Oligonucleotides and siRNAs into Mammalian Cells Mouldy Sioud Local and Systemic Delivery of siRNAs for Oligonucleotides Therapy Fumitaka Takeshita, Naomi Hokaiwado, Kimi Honma, and Takahiro Ochiya Imaging of siRNA Delivery and Silencing Anna Moore and Zdravka Medarova Recent Advances in Magnetofection and Its Potential to Deliver siRNAs in vitro Olga Mykhaylyk, Olivier Zelphati, Edelburga Hammerschmid, Martina Anton, Joseph Rosenecker and Christian Plank In vitro and in vivo Gene Silencing by Transkingdom RNAi (tkRNAi) Shuanglin Xiang, Andrew C. Keates, Johannes Fruehauf, Youxin Yang, Hongnian Guo, Thu Nguyen, and Chiang J. Li Bacterial Delivery of siRNAs: A New Approach to Solid Tumor Therapy De-Qi Xu, Ling Zhang, Dennis J. Kopecko, Lifang Gao, Yueting Shao, Baofeng Guo, and Lijing Zhao The Therapeutic Potential of LNA-modified siRNAs: Reduction of Off-target Effects by Chemical Modification of the siRNA Sequence Kees Fluiter, Olaf R. F. Mook, and Frank Baas pSM155 and pSM30 Vectors for miRNA and shRNA Expression Junzhu Wu, Akua N. Bonsra, and Guangwei Du Targeting Oncogenes with siRNAs Olaf Heidenreich Targeting Stromal-cancer Cell Interactions with siRNAs Seyedhossein Aharinejad, Mouldy Sioud, Trevor Lucas, and Dietmar Abraham Therapeutic Potential of siRNA-mediated Targeting of Urokinase Plasminogen Activator, Its Receptor, and Matrix Metalloproteinases Christopher S. Gondi and Jasti S. Rao Silencing of HIF-1a by RNA Interference in Human Glioma Cells in vitro and in vivo David L. Gillespie, Jeannette R. Flynn, Brian T. Ragel, Maria Arce-Larreta, David A. Kelly, Sheryl R. Tripp, and Randy L. Jensen RNA Interference-mediated Validation of Genes Involved in Telomere Maintenance and Evasion of Apoptosis as Cancer Therapeutic Targets Marco Folini, Marzia Pennati, and Nadia Zaffaroni Treating Respiratory Viral Diseases with Chemically Modified, Second Generation Intranasal siRNAs Sailen Barik Progress in the Therapeutic Applications of siRNAs against HIV-1 Miguel Angel Martínez Protein Components of the microRNA Pathway and Human Diseases Marjorie P. Perron and Patrick Provost Intron-mediated RNA Interference and microRNA Biogenesis Shao-Yao Ying and Shi-Lung Lin Emergence of a Complex Relationship between HIV-1 and the microRNA Pathway Dominique L. Ouellet, Isabelle Plante, Corinne Barat, Michel J. Tremblay, and Patrick Provost Synthetic microRNA Targeting Glioma-associated Antigen-1 Protein Naotake Tsuda, Takahi Mine, Constantin G. Ioannides, and David Z. Chang Therapeutic Targeting of Gene Expression by siRNA Directed against BCR-ABL Transcripts in a Patient with Imatinib-resistant Chronic Myeloid Leukemia Michael Koldehoff and Ahmet H. Elmaagacli