Progress in functional proteomics has been limited for a long time, partially caused by limitations in assay sensitivity and sample capacity; however, protein microarrays have the ability to overcome these limitations so that a highly parallel analysis of hundreds of proteins in thousands of samples is attainable.  In Protein Microarrays: Methods and Protocols, expert researchers in the field present an up-to-date collection of robust strategies in the field of protein microarrays and summarize recent advantages in the field of printing technologies, the development of suitable surface materials, as well as detection and quantification technologies.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Protein Microarrays: Methods and Protocols aims to stimulate the application and further advancement of this powerful technology in labs worldwide.

Part I: Reverse Phase Protein Arrays   1. Reverse Phase Protein Microarrays for Clinical Applications             Mariaelena Pierobon, Claudio Belluco, and Lance A. Liotta, and Emanuel F. Petricoin III   2. Impact of Blocking and Detection Chemistries on Antibody Performance for Reverse Phase Protein Arrays             Kristi Ambroz   3. Phosphoprotein Stability in Clinical Tissue and Its Relevance for Reverse Phase Protein Microarray Technology             Virginia Espina, Claudius Mueller, and Lance A. Liotta   4. Utilization of RNAi to Validate Antibodies for Reverse Phase Protein Arrays             Heiko Mannsperger, Stefan Uhlmann, Ulrike Korf, and Özgür Sahin   5. Antibody-Mediated Signal Amplification for Reverse Phase Protein Array-Based Protein Quantification             Jan C. Brase, Heiko Mannsperger, Holger Sültmann, and Ulrike Korf   6. Reverse-Phase Protein Lysate Microarray (RPA) for the Experimental Validation of Quantitative Protein Network Models             Satoshi Nishizuka   7. Characterization of Kinase Inhibitors Using Reverse Phase Protein Arrays             Georg Martiny-Baron, Dorothea Haasen, Daniel D¿Dorazio, Johannes Voshol, and Doriano Fabbro   8. Use of Formalin-Fixed and Paraffin-Embedded (FFPE) Tissues  for Diagnosis and Therapy in Routine Clinical Settings             Daniela Berg, Katharina Malinowsky, Bilge Ergin, Claudia Wolff, and Karl-Friedrich Becker   9. Producing Reverse Phase Protein Microarrays from Formalin Fixed Tissues             Claudia Wolff, Claudia Schott, Katharina Malinowsky, Daniela Berg, and Karl-Friedrich Becker   10. Use of Reverse Phase Protein Microarrays to Study Protein Expression in Leukemia: Technical and Methodological Lessons Learned             Steven M. Kornblau and Kevin C. Coombes   Part II: Antibody Microarrays   11. Antibody Microarrays as Tools for Biomarker Discovery             Marta Sanchez-Carbayo   12. Assessment of Antibody Specificity Using Suspension Bead Arrays             Jochen M. Schwenk and Peter Nilsson   13. Quantitative Analysis of Phosphoproteins Using Microspot Immunoassays             Frauke Henjes, Frank Götschel, Anika Jöcker, and Ulrike Korf   14. Robust Protein Profiling with Complex Antibody Microarrays in a Dual-Color Mode             Christoph Schröder, Mohamed S.S. Alhamdani, Kurt Fellenberg, Andrea Bauer, Anette Jacob, and Jörg D. Hoheisel   15. High-Throughput Studies of Protein Glycoforms Using Antibody-Lectin Sandwich Arrays             Brian B. Haab and Tingting Yue   16. Microspot Immunoassay-Based Analysis of Plasma Protein Profiles for Biomarker Discovery Strategies             Johanna Sonntag, Heiko Mannsperger, Anika Joecker, and Ulrike Korf   17. Recombinant Antibodies for the Generation of Antibody Arrays             Carl A.K. Borrebaeck and Christer Wingren   Part III: Protein Microarrays   18. Producing Protein Microarrays from DNA Microarrays             Oda Stoevesandt, Michael J. Taussig, and Mingyue He   19. Cell Arrays and High-Content Screening             Holger Erfle, Anastasia Eskova, Jürgen Reymann, and Vytaute Starkuviene   20. Probing Calmodulin Protein-Protein Interactions Using High Content Protein Arrays             David J. O¿Connell, Mikael Bauer, Sara Linse, and Dolores J. Cahill   21. Protein Function Microarrays for Customized Systems-Oriented Proteome Analysis             Jonathan M. Blackburn and Aubrey Shoko   22. Optimized Autoantibody Profiling on Protein Arrays             Sara L. O¿Kane, John K. O¿Brien, and Dolores J. Cahill   Part IV: Sample Immobilization Strategies   23. Inkjet Printing for the Production of Protein Microarrays             Iain McWilliam, Marisa Chong Kwan, and Duncan Hall   24. Impact of Substrates for Probe Immobilization ...