Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

1. Targeted Deletion of Hsf1, 2, and 4 Genes in Mice             Xiongjie Jin, Binnur Eroglu, Demetrius Moskophidis, and Nahid F. Mivechi   2. The Role Of Heat Shock Factors in Stress-Induced Transcription                  Yue Zhang, Shiuh-Dih Chou, Ayesha Murshid, Thomas L. Prince, Sheila Schreiner, Mary Ann Stevenson, and Stuart K. Calderwood   3. Hsp90 and Client Protein Maturation             Natalie Wayne, Parul Mishra, and Daniel N. Bolon   4. The Role of p23, Hop, Immunophilins, and Other Co-Chaperones in Regulating Hsp90 Function             Marc B. Cox and Jill L. Johnson   5. Detecting Hsp90 Phosphorylation             Mehdi Mollapour and Len Neckers   6. Role of Molecular Chaperones in Biogenesis of the Protein Kinome             Atin K. Mandal, Maria A. Theodoraki, Nadinath B. Nillegoda, and Avrom J. Caplan   7. Nucleotide Exchange Factors for Hsp70 Chaperones             Heike Rampelt, Matthias P. Mayer, and Bernd Bukau   8. Reconstitution of CHIP E3 Ubiquitin Ligase Activity             Hong Yu Ren, Cam Patterson, Douglas M. Cyr, and Meredith F.N. Rosser   9. Structure-Functions of HspB1 (Hsp27)             Andre-Patrick Arrigo   10. Combined Lentiviral and RNAi Technologies for the Delivery and Permanently Silencing of the hsp25 Gene             Punit Kaur, Ganachari M. Nagaraja, Khalid Bin Abdulrahman, and Alexzander Asea   11. Quantification of HSP27 and HSP70 Molecular Chaperone Activities             Jessica Gobbo, Caroline Gaucher-Di-Stasio, Stephanie Weidmann, Jean Guzzo, and Carmen Garrido   12. Measuring Hsp72 (HSPA1A) by Indirect Sandwich ELISA             H. Elyse Ireland and John H.H. Williams   13. Analysis of Heat Shock Protein Localization Using Flow Cytometry             Nina C. Dempsey, Francesca Leoni, Christine Hoyle, and John. H.H. Williams   14. Quantitation of Heat Shock Proteins in Clinical Samples Using Mass Spectrometry             Punit Kaur, Khalid Bin Abdulrahman, and Alexzander Asea   15. Bioinformatic Approach to Identify Chaperone Pathway Relationship from Large-Scale Interaction Networks             Yunchen Gong, Zhaolei Zhang, and Walid A. Houry   16. Hsp70: Anti-Apoptotic and Tumorigenic Protein             Anne-Laure Rerole, Gaetan Jego, and Carmen Garrido   17. Determination of Cell Survival or Death             Alexander  E. Kabakov, Vladimir A. Kudryavtsev, and Vladimir L. Gabai   18. Immunohistochemistry of Human Hsp60 in Health and Disease: From Autoimmunity to Cancer             Francesco Cappello, Everly Conway de Macario, Giovanni Zummo, and Alberto J.L. Macario   19. Preparation of a Heat Shock Proteins 70-Based Vaccine from DC-Tumor Fusion Cells             Desheng Weng, Stuart K. Calderwood, and Jianlin Gong   20. Isolation of Heat Shock Protein Complexes             Fernando D. Cuello-Carrion, Mariel A. Fanelli, Niubys Cayado-Gutierrez, Gisela Castro, and Daniel R. Ciocca   21. Enhancing Antigen Cross-Presentation and T-Cell Priming by Complexing Protein Antigen to Recombinant Large Heat Shock Protein             Xiang-Yang Wang, Huanfa Yi, Xiaofei Yu, Damin Zuo, and John R. Subjeck   22. Investigating Receptors for Extracellular Heat Shock Proteins             Ayesha Murshid, Jimmy Theriault, Jianlin Gong, and Stuart K. Calderwood   23. Analysis of Cellular Migration Using a Two-Chamber Methodology             Michelle M. Kouspou and John T. Price