Mitochondrial Bioenergetics

Methods and Protocols, Methods in Molecular Biology 810

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Bibliografische Daten
ISBN/EAN: 9781617793813
Sprache: Englisch
Umfang: xi, 333 S., 58 s/w Illustr., 25 farbige Illustr.,
Auflage: 1. Auflage 2012
Einband: gebundenes Buch

Beschreibung

Inhaltsangabe1 - Overview of Mitochondrial Bioenergetics (Vitor M. C. Madeira)2 - Evaluation of Respiration with Clark Type Electrode in Isolated Mitochondria and Permeabilized Animal Cells (Ana M. Silva and Paulo J. Oliveira)3 - High-Resolution Respirometry.  OXPHOS Protocols for Human Cells and Permeabilized Fibres from Small Biopsies of Human Muscle (Dominik Pesta and Erich Gnaiger)4 - High Throughput Analysis of Mitochondrial Oxygen Consumption (James Hynes, Rachel L. Swiss and Yvonne Will)5 - Modulation of Cellular Respiration by Endogenously Produced Nitric Oxide in Rat Hippocampal Slices(Ana Ledo, Rui M. Barbosa and João Laranjinha)6 - Mitochondrial Membrane Potential (DY) Fluctuations Associated with the Metabolic States of Mitochondria (Carlos M. Palmeira and Anabela P. Rolo)7 - Safranine as a Fluorescent Probe for the Evaluation of Mitochondrial Membrane Potential in Isolated Organelles and Permeabilized Cells (Tiago R. Figueira, Daniela R. Melo, Aníbal E. Vercesi and Roger F. Castilho)8 - Fluorescence Measurement of Mitochondrial Membrane Potential Changes in Cultured Cells (D. Nicholls)9 - Phenomenological Kinetic and Control Analysis of Oxidative Phosphorylation in Isolated Mitochondria (Vilmante Borutaite and Rasa Baniene)10 - Expression of Uncoupling Proteins in a Mammalian Cell Culture System (HEK293) and Assessment of their Protein Function (Martin  Jastroch)11 - Measurement of Proton Leak and Electron Leak in Isolated Mitochondria (Charles Affourtit, Casey L. Quinlan and Martin D. Brand)12 - Relation between Mitochondrial Membrane Potential and ROS Formation (Jan Suski, Magdalena Lebiedzinska, Massimo Bonora, Paolo Pinton, Jerzy Duszynski and Mariusz R. Wieckowski) 13 - Use of a Calcium-Sensitive Electrode for Studies on Mitochondrial Calcium Transport (António J. Moreno and Joaquim A. F. Vicente) 14 - Imaging Mitochondrial Calcium Signalling with Fluorescent Probes and Single or Two Photon Confocal Microscopy (Sean M. Davidson and Michael R. Duchen) 15 - Mitochondrial Permeability Transition Pore and Calcium Handling (Renee Wong, Charles Steenbergen and Elizabeth Murphy)16- Imaging of Mitochondrial pH Using SNARF-1(Venkat K. Ramshesh and John J. Lemasters)17- Redox Equivalents and Mitochondrial Bioenergetics(James R. Roede, Young-Mi Go and Dean P. Jones)18-NMR Methodologies for Studying Mitochondrial Bioenergetics(Tiago C. Alves, Ivana Jarak and Rui A. Carvalho)19-Computational Modeling of Mitochondrial Function(Sonia Cortassa and Miguel A. Aon)

Inhalt

1 - Overview of Mitochondrial Bioenergetics (Vitor M. C. Madeira) 2 - Evaluation of Respiration with Clark Type Electrode in Isolated Mitochondria and Permeabilized Animal Cells (Ana M. Silva and Paulo J. Oliveira) 3 - High-Resolution Respirometry.  OXPHOS Protocols for Human Cells and Permeabilized Fibres from Small Biopsies of Human Muscle (Dominik Pesta and Erich Gnaiger) 4 - High Throughput Analysis of Mitochondrial Oxygen Consumption (James Hynes, Rachel L. Swiss and Yvonne Will) 5 - Modulation of Cellular Respiration by Endogenously Produced Nitric Oxide in Rat Hippocampal Slices(Ana Ledo, Rui M. Barbosa and João Laranjinha) 6 - Mitochondrial Membrane Potential (DY) Fluctuations Associated with the Metabolic States of Mitochondria (Carlos M. Palmeira and Anabela P. Rolo) 7 - Safranine as a Fluorescent Probe for the Evaluation of Mitochondrial Membrane Potential in Isolated Organelles and Permeabilized Cells (Tiago R. Figueira, Daniela R. Melo, Aníbal E. Vercesi and Roger F. Castilho) 8 - Fluorescence Measurement of Mitochondrial Membrane Potential Changes in Cultured Cells (D. Nicholls) 9 - Phenomenological Kinetic and Control Analysis of Oxidative Phosphorylation in Isolated Mitochondria (Vilmante Borutaite and Rasa Baniene) 10 - Expression of Uncoupling Proteins in a Mammalian Cell Culture System (HEK293) and Assessment of their Protein Function (Martin  Jastroch) 11 - Measurement of Proton Leak and Electron Leak in Isolated Mitochondria  (Charles Affourtit, Casey L. Quinlan and Martin D. Brand) 12 - Relation between Mitochondrial Membrane Potential and ROS Formation (Jan Suski, Magdalena Lebiedzinska, Massimo Bonora, Paolo Pinton, Jerzy Duszynski and Mariusz R. Wieckowski)   13 - Use of a Calcium-Sensitive Electrode for Studies on Mitochondrial Calcium Transport (Antonio J. Moreno and Joaquim A. F. Vicente)   14 - Imaging Mitochondrial Calcium Signalling with Fluorescent Probes and Single or Two Photon Confocal Microscopy (Sean M. Davidson and Michael R. Duchen)   15 - Mitochondrial Permeability Transition Pore and Calcium Handling  (Renee Wong, Charles Steenbergen and Elizabeth Murphy)  16 - Imaging of Mitochondrial pH Using SNARF-1 (Venkat K. Ramshesh and John J. Lemasters)  17 - Redox Equivalents and Mitochondrial Bioenergetics (James R. Roede, Young-Mi Go and Dean P. Jones)  18 - NMR Methodologies for Studying Mitochondrial Bioenergetics  (Tiago C. Alves, Ivana Jarak and Rui A. Carvalho)                    16 - Imaging of Mitochondrial pH Using SNARF-1 (Venkat K. Ramshesh and John J. Lemasters)  17 - Redox Equivalents and Mitochondrial Bioenergetics (James R. Roede, Young-Mi Go and Dean P. Jones)  18 - NMR Methodologies for Studying Mitochondrial Bioenergetics  (Tiago C. Alves, Ivana Jarak and Rui A. Carvalho)                17 - Redox Equivalents and Mitochondrial Bioenergetics (James R. Roede, Young-Mi Go and Dean P. Jones)  18 - NMR Methodologies for Studying Mitochondrial Bioenergetics  (Tiago C. Alves, Ivana Jarak and Rui A. Carvalho)            18 - NMR Methodologies for Studying Mitochondrial Bioenergetics  (Tiago C. Alves, Ivana Jarak and Rui A. Carvalho)       (Tiago C. Alves, Ivana Jarak and Rui A. Carvalho)              16 - Imaging of Mitochondrial pH Using SNARF-1 (Venkat K. Ramshesh and John J. Lemasters)  17 - Redox Equivalents and Mitochondrial Bioenergetics (James R. Roede, Young-Mi Go and Dean P. Jones)  18 - NMR Methodologies for Studying Mitochondrial Bioenergetics  (Tiago C. Alves, Ivana Jarak and Rui A. Carvalho)                17 - Redox Equivalents and Mitochondrial Bioenergetics (James R. Roede, Young-Mi Go and Dean P. Jones)  18 - NMR Methodologies for Studying Mitochondrial Bioenergetics  (T ...