InhaltsangabePart 1. Overview of Astrocyte Biology1. Astrocytes:  Multi-talented Stars of the Central Nervous System(Ransom-B)2. Neurogenic Astrocytes and their Glycoconjugates: not just "Glue" Anymore (Steindler-D)3. Heterogeneity of Astrocytic Form and Function (Goldman-S/ Nedergaard-M) Part 2. Isolation and Culture of Astrocytes4. Preparation of Mixed Glial Cultures from Postnatal Rat Brain(DeVellis-J)5. Generation of Primary Astrocyte Cultures Devoid of Contaminating Microglia (Hewett-S6. Derivation of Microglia-free Astrocyte Cultures from Neural Stem Cells (Milner-R)7. Isolation and Culture of Spinal Cord Astrocytes (Miller-R8. Isolation and Culture of Astrocytes from the Retina and Optic Nerve (Lukas-T)9. Primary Cultures of Astrocytes from Fetal Bovine Brain (Ballarin-C)10. Isolation and Cultivation of Porcine Astrocytes (Bobilya-D)11. Isolation and Culture of Human Astrocytes (Sharif-A/ Prevot-V)12. Cell Culturing of C. elegans Glial Cells for the Assessment of Cytosolic Ca2+ Dynamics (Parpura-V) Part 3. Analysis of Astrocyte Functions In Vitro13. Astrocyte Polarization and Wound Healing in Culture:Studying Cell Adhesion Molecules (Carbonetto-S) 14. An in Vitro Trauma Model to Study Rodent and Human Astrocyte Reactivity (Wanner-I)15.  Use of Gel Zymography to Examine Matrix Metalloproteinase (gelatinase) Expression in Brain Tissue or in Primary Glial Cultures (del Zoppo-G)16.  ELISA Methodology to Quantify Astrocyte Production of Cytokines/Chemokines In Vitro (Benveniste-T)17.  Induction of Nitric Oxide Synthase-2 Expression and Measurement of Nitric Oxide Production in Enriched Primary Cortical Astrocyte Cultures (Hewett-J)18.  Examining  Potassium Channel Function in Astrocytes (Olsen-M)19.  Connexin Based Channels in Astrocytes: How to Study their Properties (Giaume-C)20.  Isotope-based Quantitation of Uptake, Release and Metabolism of Glutamate and Glucose in Cultured Astrocytes (Hertz-L) Part 4. Co-culture systems to analyse astrocyte interactions with other cell types21.  An in Vitro Model of Reactive Astrogliosis and its Effect on Neuronal Growth (Geller-H)22.  A Neuron-Astrocyte Co-Culture System to Investigate Astrocyte Secreted Factors in Mouse Neuronal Development (Murai-K) 23.  Co-cultures of Neurons and Astrocytes as a Model for Examining Hypoxia-induced Neuronal Death (Rempe-D).24.  Use of Astrocyte-Microglial Co-cultures to Examine the Regulatory Influence of Astrocytes on Microglial Activation (Milner-R)25.  Astrocyte-Schwann Cell Co-Culture Systems (Fawcett-J)26.  An in Vitro Assay to Examine Oligodendrocyte Precursor Cell Migration on Astrocytes (Fawcett-J)27.  Investigation of Astrocyte-Oligodendrocyte Interactions in Human Cultures (John-G)28.  An Improved in vitro Blood-Brain Barrier Model: Rat Brain Endothelial Cells Co-Cultured with Astrocytes (Abbott-J) 29.  In Vitro Models of the Blood-Brain Barrier (Abbruscato-T)30.  An in Vitro Model of Ischemic Stroke (Abbruscato-T)31.  Pericyte Co-Culture Models to Study Astrocyte, Pericyte, and Endothelial Cell Interactions (Dore-Duffy-P)Part 5. Analysis of Astrocyte Functions In Vivo32.  Reprogramming of Postnatal Astroglia of the Mouse Neocortex into Functional Synapse-Forming Neurons (Berninger-B)33.  Assessment of Glial Function in the in Vivo Retina (Newman-E)34.  Imaging in Vivo Astrocyte/Endothelial Cell Interactions at the Blood-Brain Barrier (Willis-C)35.  Transgenic Techniques for Cell Ablation or Molecular Deletion to Investigate Functions of Astrocytes and Other GFAP-Expressing Cell Types (Sofroniew-M)36.  Use of Cre-Lox Technology to Analyze Integrin Functions in Astrocytes (McCarty-J)37.  In Vivo Imaging of Ca2+ Signaling in Astrocytes Using Two Ph

Astrocytes: Methods and Protocols Part 1. Overview of Astrocyte Biology 1. Astrocytes:  Multi-talented Stars of the Central Nervous System(Ransom-B) 2. Neurogenic Astrocytes and their Glycoconjugates: not just ''Gluea¿ Anymore (Steindler-D) 3. Heterogeneity of Astrocytic Form and Function (Goldman-S/ Nedergaard-M)   Part 2. Isolation and Culture of Astrocytes4. Preparation of Mixed Glial Cultures from Postnatal Rat Brain(DeVellis-J) 5. Generation of Primary Astrocyte Cultures Devoid of Contaminating Microglia (Hewett-S )6. Derivation of Microglia-free Astrocyte Cultures from Neural Stem Cells (Milner-R) 7. Isolation and Culture of Spinal Cord Astrocytes (Miller-R )8. Isolation and Culture of Astrocytes from the Retina and Optic Nerve (Lukas-T )9. Primary Cultures of Astrocytes from Fetal Bovine Brain (Ballarin-C) 10. Isolation and Cultivation of Porcine Astrocytes (Bobilya-D) 11. Isolation and Culture of Human Astrocytes (Sharif-A/ Prevot-V) 12. Cell Culturing of C. elegans Glial Cells for the Assessment of Cytosolic Ca2+ Dynamics (Parpura-V)   Part 3. Analysis of Astrocyte Functions In Vitro 13. Astrocyte Polarization and Wound Healing in Culture:Studying Cell Adhesion Molecules (Carbonetto-S) 14. An in Vitro Trauma Model to Study Rodent and Human Astrocyte Reactivity (Wanner-I) 15.  Use of Gel Zymography to Examine Matrix Metalloproteinase (gelatinase) Expression in Brain Tissue or in Primary Glial Cultures (del Zoppo-G) 16.  ELISA Methodology to Quantify Astrocyte Production of Cytokines/Chemokines In Vitro (Benveniste-T) 17.  Induction of Nitric Oxide Synthase-2 Expression and Measurement of Nitric Oxide Production in Enriched Primary Cortical Astrocyte Cultures (Hewett-J) 18.  Examining  Potassium Channel Function in Astrocytes (Olsen-M) 19.  Connexin Based Channels in Astrocytes: How to Study their Properties (Giaume-C) 20.  Isotope-based Quantitation of Uptake, Release and Metabolism of Glutamate and Glucose in Cultured Astrocytes (Hertz-L)   Part 4. Co-culture systems to analyse astrocyte interactions with other cell types21.  An in Vitro Model of Reactive Astrogliosis and its Effect on Neuronal Growth (Geller-H) 22.  A Neuron-Astrocyte Co-Culture System to Investigate Astrocyte Secreted Factors in Mouse Neuronal Development (Murai-K) 23.  Co-cultures of Neurons and Astrocytes as a Model for Examining Hypoxia-induced Neuronal Death (Rempe-D). 24.  Use of Astrocyte-Microglial Co-cultures to Examine the Regulatory Influence of Astrocytes on Microglial Activation (Milner-R) 25.  Astrocyte-Schwann Cell Co-Culture Systems (Fawcett-J) 26.  An in Vitro Assay to Examine Oligodendrocyte Precursor Cell Migration on Astrocytes (Fawcett-J) 27.  Investigation of Astrocyte-Oligodendrocyte Interactions in Human Cultures (John-G) 28.  An Improved in vitro Blood-Brain Barrier Model: Rat Brain Endothelial Cells Co-Cultured with Astrocytes (Abbott-J) 29.  In Vitro Models of the Blood-Brain Barrier (Abbruscato-T) 30.  An in Vitro Model of Ischemic Stroke (Abbruscato-T) 31.  Pericyte Co-Culture Models to Study Astrocyte, Pericyte, and Endothelial Cell Interactions (Dore-Duffy-P)   Part 5. Analysis of Astrocyte Functions In Vivo32.  Reprogramming of Postnatal Astroglia of the Mouse Neocortex into Functional Synapse-Forming Neurons (Berninger-B) 33.  Assessment of Glial Function in the in Vivo Retina (Newman-E) 34.  Imaging in Vivo Astrocyte/Endothelial Cell Interactions at the Blood-Brain Barrier (Willis-C) 35.  Transgenic Techniques for Cell Ablation or Molecular Deletion to Investigate Functions of Astrocytes and Other GFAP-Expressing Cell Types (Sofroniew-M) 36.  Use of Cre-Lox technology to analyze integrin functions in astrocytes (McCarty-J) 37.  In Vivo Imaging of Ca2+ Signaling in Astrocytes Using Two Photon Laser Scanning Fluorescent Microscopy (Ding-S)