Inhaltsangabe1. Mucin Methods: Genes Encoding Mucins And Their Genetic Variation With A Focus On Gel Forming Mucins Karine Rousseau and Dallas M. Swallow2. Gel-forming and Cell-associated Mucins - preparation for Structural and Functional Studies Julia R. Davies, Claes Wickström and David J. Thornton 3. Detecting, Visualising and Quantifying MucinsCeri A. Harrop, David J. Thornton and Michael A. McGuckin4. Mass Spectrometric Analysis of Mucin Core ProteinsMehmet Kesimer and John K. Sheehan5. O-Glycoprotein Biosynthesis:  Site Localization by Edman Degradation and Site Prediction Based on Random Peptide SubstratesThomas A. Gerken6. Analysis of Assembly of Secreted Mucins Malin E.V. Johansson and Gunnar C. Hansson7. MUC1 Membrane Trafficking: Protocols for Assessing Biosynthetic Delivery, Endocytosis, Recycling and Release Through ExosomesFranz-Georg Hanisch, Carol L. Kinlough, Simon Staubach, Rebecca P. Hughey8.Glycomic Work-flow for Analysis of Mucin O-linked OligosaccharidesCatherine A. Hayes, Szilard Nemes, Samah Issa, Chunseng Jin,  and Niclas G. Karlsson9. O-Glycomics: Profoliling and Structural Analysis of Mucin-type O-linked GlycansIsabelle Breloy10. O-Glycoproteomics: Site-specific O-glycoprotein Analysis by CID/ETD Electrospray Ionization Tandem Mass Spectrometry and Top-Down Glycoprotein Sequencing by In-Source Decay MALDI Mass Spectrometry Franz-Georg Hanisch11. Analysing Mucin Degradation  Stephen D. Carrington, Jane A. Irwin, Li Liu, Pauline Rudd, Elizabeth Matthews, and Anthony P. Corfield Stephen D. Carrington, Jane A. Irwin, Li Liu, Pauline Rudd, Elizabeth Matthews, and Anthony P. Corfield Stephen D. Carrington, Jane A. Irwin, Li Liu, Pauline Rudd, Elizabeth Matthews, and Anthony P. Corfield Stephen D. Carrington, Jane A. Irwin, Li Liu, Pauline Rudd, Elizabeth Matthews, and Anthony P. Corfield Stephen D. Carrington, Jane A. Irwin, Li Liu, Pauline Rudd, Elizabeth Matthews, and Anthony P. Corfield Stephen D. Carrington, Jane A. Irwin, Li Liu, Pauline Rudd, Elizabeth Matthews, and Anthony P. Corfield Stephen D. Carrington, Jane A. Irwin, Li Liu, Pauline Rudd, Elizabeth Matthews, and Anthony P. Corfield Stephen D. Carrington, Jane A. Irwin, Li Liu, Pauline Rudd, Elizabeth Matthews, and Anthony P. Corfield Stephen D. Carrington, Jane A. Irwin, Li Liu, Pauline Rudd, Elizabeth Matthews, and Anthony P. Corfield Stephen D. Carrington, Jane A. Irwin, Li Liu, Pauline Rudd, Elizabeth Matthews, and Anthony P. Corfield12.  Assessment of Mucus Thickness and Production In SituLena Holm and Mia Phillipson13. Preservation of Mucus in Histrological Sections, Immunostaining of Mucins in Fixed Tissue and Localization of Bacteria with FISHMalin E.V. Johansson and Gunnar C. Hansson14. In vitro Measurements of Mucus Secretion by Colon ExplantsJenny K. Gustafsson, Henrik Sjövall and Gunnar C. Hansson15. Establishment of Respiratory Air-Liquid Interface Cultures and their Use in Studying Mucin Production, Secretion, and FunctionDavid R. Hill and Brian ButtonDavid R. Hill and Brian ButtonDavid R. Hill and Brian ButtonDavid R. Hill and Brian Button16. Studying Mucin Secretion from Human Bronchial Epithelial Cell Primary Cultures Lubna H. Abdullah, Cedric Wolber, Mehment Kesimer, John K. Sheehan, and C. William Davis17. Assessment of Intracellular Mucin Content In VivoLucia Piccotti, Burton F. Dickey, Christopher M. Evans18. Techniques for

Table of ContentsChapter Title     1 Mucin Methods: Genes Encoding Mucins And Their Genetic Variation With A Focus On Gel Forming Mucins   2 Gel-forming and Cell-associated Mucins - preparation for Structural and Functional Studies   3 Detecting, Visualising and Quantifying Mucins   4 Mass Spectrometric Analysis of Mucin Core Proteins   5 O-Glycoprotein Biosynthesis:  Site Localization by Edman Degradation and Site Prediction Based on Random Peptide Substrates   6 Analysis of Assembly of Secreted Mucins   7 MUC1 Membrane Trafficking: Protocols for Assessing Biosynthetic Delivery, Endocytosis, Recycling and Release Through Exosomes   8 Glycomic Work-flow for Analysis of Mucin O-linked Oligosaccharides    9 O-Glycomics: Profiling and Structural Analysis of Mucin-type O-linked Glycans   10 O-Glycoproteomics: Site-specific O-glycoprotein Analysis by CID/ETD Electrospray Ionization Tandem Mass Spectrometry and Top-Down Glycoprotein Sequencing by In-Source Decay MALDI Mass Spectrometry   11 Analysing Mucin Degradation   12 Assessment of Mucus Thickness and Production In Situ   13 Preservation of Mucus in Histological Sections, Immunostaining of Mucins in Fixed Tissue and Localization of Bacteria with FISH   14 In vitro Measurements of Mucus Secretion by Colon Explants   15 Establishment of Respiratory Air-Liquid Interface Cultures and their Use in Studying Mucin Production, Secretion, and Function   16 Studying Mucin Secretion from Human Bronchial Epithelial Cell Primary Cultures   17 Assessment of Intracellular Mucin Content In Vivo 18 Techniques for Assessment of Interactions of Mucins with Microbes and Parasites In Vitro and In Vivo   19 Assessing Mucin Expression and Function in Human Ocular Surface Epithelia In Vivo and In Vitro     Chapter Title     1 Mucin Methods: Genes Encoding Mucins And Their Genetic Variation With A Focus On Gel Forming Mucins   2 Gel-forming and Cell-associated Mucins - preparation for Structural and Functional Studies   3 Detecting, Visualising and Quantifying Mucins   4 Mass Spectrometric Analysis of Mucin Core Proteins   5 O-Glycoprotein Biosynthesis:  Site Localization by Edman Degradation and Site Prediction Based on Random Peptide Substrates   6 Analysis of Assembly of Secreted Mucins   7 MUC1 Membrane Trafficking: Protocols for Assessing Biosynthetic Delivery, Endocytosis, Recycling and Release Through Exosomes   8 Glycomic Work-flow for Analysis of Mucin O-linked Oligosaccharides    9 O-Glycomics: Profiling and Structural Analysis of Mucin-type O-linked Glycans   10 O-Glycoproteomics: Site-specific O-glycoprotein Analysis by CID/ETD Electrospray Ionization Tandem Mass Spectrometry and Top-Down Glycoprotein Sequencing by In-Source Decay MALDI Mass Spectrometry   11 Analysing Mucin Degradation   12 Assessment of Mucus Thickness and Production In Situ   13 Preservation of Mucus in Histological Sections, Immunostaining of Mucins in Fixed Tissue and Localization of Bacteria with FISH   14 In vitro Measurements of Mucus Secretion by Colon Explants   15 Establishment of Respiratory Air-Liquid Interface Cultures and their Use in Studying Mucin Production, Secretion, and Function   16 Studying Mucin Secretion from Human Bronchial Epithelial Cell Primary Cultures   17 Assessment of Intracellular Mucin Content In Vivo 18 Techniques for Assessment of Interactions of Mucins with Microbes and Parasites In Vitro and In Vivo   19 Assessing Mucin Expression and Function in Human Ocular Surface Epithelia In Vivo and In Vitro