Ancient DNA

Methods and Protocols, Methods in Molecular Biology 840

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Bibliografische Daten
ISBN/EAN: 9781617795152
Sprache: Englisch
Umfang: xii, 247 S., 14 s/w Illustr., 12 farbige Illustr.,
Auflage: 1. Auflage 2012
Einband: gebundenes Buch

Beschreibung

InhaltsangabePrefaceB. Shapiro* and M. Hofreiter Chapter 1:  Setting Up An Ancient DNA LaboratoryT. L. Fulton* Chapter 2: A Phenol-Chloroform Protocol for Extracting DNA from Ancient SamplesR. Barnett* and G. Larson Chapter 3: DNA Extraction of Ancient Animal Hard Tissue Samples via Adsorption to Silica ParticlesN. Rohland* Chapter 4: Case Study: Recovery of ancient nuclear DNA from Toe Pads of the Extinct Passenger Pigeon  T. L. Fulton*, S. M. Wagner and B. Shapiro Chapter 5: Extraction of DNA from PaleofecesM. Kuch and H. Poinar* Chapter 6: DNA Extraction from Keratin and ChitinP. F. Campos, M. T. P. Gilbert* Chapter 7: Case Study:  Ancient sloth DNA Recovered from Hairs Preserved in Paleofeces A.A. Clack*, R.D.E. MacPhee, H.N. Poinar Chapter 8: Ancient DNA Extraction from Soils and SedimentsJ. Haile* Chapter 9: DNA Extraction from Fossil EggshellC. L. Oskam and M. Bunce* Chapter 10: Ancient DNA Extraction from PlantsL. Kistler* Chapter 11: DNA Extraction from Formalin-fixed MaterialP. F. Campos, M. T. P. Gilbert* Chapter 12: Case Study: Ancient DNA recovered from Pleistocene-age Remains of a Florida Armadillo B. Letts and B. Shapiro* Chapter 13: Non-destructive DNA Extraction from Museum SpecimensM. Hofreiter* Chapter 14: Case Study: Using a Non-destructive DNA Extraction Method to Generate mtDNA Sequences from Historical Chimpanzee Specimens E. Mohandesan, S. Prost, M. Hofreiter* Chapter 15: PCR Amplification, Cloning and Sequencing of Ancient DNAT. L. Fulton* and M. Stiller Chapter 16: Quantitative Real-time PCR in aDNA Research M. Bunce*, C. L. Oskam, M. E. Allentoft Chapter 17: Multiplex PCR Amplification of Ancient DNAM. Stiller*, T. L. Fulton Chapter 18: Preparation of Next-generation Sequencing Libraries from Damaged DNAA. W. Briggs*, P. Heyn Chapter 19: Generating Barcoded Libraries for Multiplex High-throughput Sequencing  M. Knapp*, M. Stiller, M. Meyer Chapter 20: Case Study: Targeted high-throughput Sequencing of Mitochondrial Genomes from Extinct Cave Bears via Direct Multiplex PCR Sequencing (DMPS)M. Stiller* Chapter 21: Target Enrichment via DNA Hybridization CaptureS. Horn* Chapter 22: Case Study: Enrichment of Ancient Mitochondrial DNA by Hybridization Capture  S. Horn* Chapter 23: Analysis of High-throughput Ancient DNA Sequencing DataM. Kircher* Chapter 24: Phylogenetic Analysis of Ancient DNA using BEASTS. Y. W. Ho* E. Mohandesan, S. Prost, M. Hofreiter* Chapter 15: PCR Amplification, Cloning and Sequencing of Ancient DNAT. L. Fulton* and M. Stiller Chapter 16: Quantitative Real-time PCR in aDNA Research M. Bunce*, C. L. Oskam, M. E. Allentoft Chapter 17: Multiplex PCR Amplification of Ancient DNAM. Stiller*, T. L. Fulton Chapter 18: Preparation of Next-generation Sequencing Libraries from Damaged DNAA. W. Briggs*, P. Heyn Chapter 19: Generating Barcoded Libraries for Multiplex High-throughput Sequencing  M. Knapp*, M. Stiller, M. Meyer Chapter 20: Case Study: Targeted high-throughput Sequencing of Mitochondrial Genomes from Extinct Cave Bears via Direct Multiplex PCR Sequencing (DMPS)M. Stiller* Chapter 21: Target Enrichment via DNA Hybridization CaptureS. Horn* Chapter 22: Case Study: Enrichment of Ancient Mitochondrial DNA by Hybridization Capture  S. Horn* Chapte

Inhalt

Preface B. Shapiro* and M. Hofreiter   Chapter 1:  Setting Up An Ancient DNA LaboratoryT. L. Fulton*   Chapter 2: A Phenol-Chloroform Protocol for Extracting DNA from Ancient Samples R. Barnett* and G. Larson   Chapter 3: DNA Extraction of Ancient Animal Hard Tissue Samples via Adsorption to Silica Particles N. Rohland*   Chapter 4: ­­Case Study: Recovery of ancient nuclear DNA from Toe Pads of the Extinct Passenger Pigeon   T. L. Fulton*, S. M. Wagner and B. Shapiro   Chapter 5: Extraction of DNA from Paleofeces M. Kuch and H. Poinar*   Chapter 6: DNA Extraction from Keratin and Chitin P. F. Campos, M. T. P. Gilbert*   Chapter 7: Case Study:  Ancient sloth DNA Recovered from Hairs Preserved in Paleofeces   A.A. Clack*, R.D.E. MacPhee, H.N. Poinar   Chapter 8: Ancient DNA Extraction from Soils and Sediments J. Haile*   Chapter 9: DNA Extraction from Fossil Eggshell C. L. Oskam and M. Bunce*   Chapter 10: Ancient DNA Extraction from Plants L. Kistler*   Chapter 11: DNA Extraction from Formalin-fixed Material P. F. Campos, M. T. P. Gilbert*   Chapter 12: Case Study: Ancient DNA recovered from Pleistocene-age Remains of a Florida Armadillo   B. Letts and B. Shapiro*   Chapter 13: Non-destructive DNA Extraction from Museum Specimens M. Hofreiter*   Chapter 14: Case Study: Using a Non-destructive DNA Extraction Method to Generate mtDNA Sequences from Historical Chimpanzee Specimens E. Mohandesan, S. Prost, M. Hofreiter*   Chapter 15: PCR Amplification, Cloning and Sequencing of Ancient DNA T. L. Fulton* and M. Stiller   Chapter 16: Quantitative Real-time PCR in aDNA Research   M. Bunce*, C. L. Oskam, M. E. Allentoft   Chapter 17: Multiplex PCR Amplification of Ancient DNA M. Stiller*, T. L. Fulton   Chapter 18: Preparation of Next-generation Sequencing Libraries from Damaged DNA A. W. Briggs*, P. Heyn   Chapter 19: Generating Barcoded Libraries for Multiplex High-throughput Sequencing   M. Knapp*, M. Stiller, M. Meyer   Chapter 20: Case Study: Targeted high-throughput Sequencing of Mitochondrial Genomes from Extinct Cave Bears via Direct Multiplex PCR Sequencing (DMPS) M. Stiller*   Chapter 21: Target Enrichment via DNA Hybridization Capture S. Horn*   Chapter 22: Case Study: Enrichment of Ancient Mitochondrial DNA by Hybridization Capture   S. Horn*   Chapter 23: Analysis of High-throughput Ancient DNA Sequencing Data M. Kircher*  Chapter 24: Phylogenetic Analysis of Ancient DNA using BEASTS. Y. W. Ho*   E. Mohandesan, S. Prost, M. Hofreiter*   Chapter 15: PCR Amplification, Cloning and Sequencing of Ancient DNA T. L. Fulton* and M. Stiller   Chapter 16: Quantitative Real-time PCR in aDNA Research   M. Bunce*, C. L. Oskam, M. E. Allentoft   Chapter 17: Multiplex PCR Amplification of Ancient DNA M. Stiller*, T. L. Fulton   Chapter 18: Preparation of Next-generation Sequencing Libraries from Damaged DNA A. W. Briggs*, P. Heyn   Chapter 19: Generating Barcoded Libraries for Multiplex High-throughput Sequencing   M. Knapp*, M. Stiller, M. Meyer   Chapter 20: Case Study: Targeted high-throughput Sequencing of Mitochondrial Genomes from Extinct Cave Bears via Direct Multiplex PCR Sequencing (DMPS) M. Stiller*   Chapter 21: Target Enrichment via DNA Hybridization Capture S. Horn*   Chapter 22: Case Study: Enrichment of Ancient Mitochondrial DNA by Hybridization Capture   S. Horn*   Chapter 23: Analysis of High-throughput Ancient DNA Sequencing Data M. Kircher*  Chapter 24: Phylogenetic Analysis of Ancient DNA using BEASTS. Y. W. Ho*   T. L. Fulton*   Chapter 2: A Phenol-Chloroform Protocol for Extracting DNA from Ancient Samples R. Barnett* and G. Larson   Chapter 3: DNA Extrac ...