Despite many technological challenges faced by the xenotransplantation field, many major advances have been made in the last two decades. The field seeks to overcome the limitations and difficulties in organ procurement, which also apply to human cells and tissues, and facilitate the development of new therapies based on cell and engineered-tissue. Xenogeneic cells are simpler than solid organs and seem to pose less hurdles to attain long-term graft survival. In, Xenotransplantation: Methods and Protocols expert researchers study characterizations of xenogeneic interactions at the cellular and molecular levels and describe the use of relevant small-animal and pig-to-primate models. Related ethical and legal considerations are also covered. Written in the highly successful Methods in Molecular Biology series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory. Thorough and intuitive, Xenotransplantation: Methods and Protocols aids scientists in continuing to study xenotransplantation and its multiple aspects.

Inhaltsangabe1. Xenotransplantation: An Overview of the FieldVeronica Tisato and Emanuele Cozzi2. Cloning and Expression Analyses of Pig GenesMireia Uribe-Herranz and Cristina Costa3. Basic Analyses of Proteins of Interest for XenotransplantationMireia Uribe-Herranz and Cristina Costa4. Studies on Carbohydrate XenoantigensDale Christiansen, Effie Mouhtouris, Paul A. Ramsland, and Mauro S.  Sandrin5. Xenoantibodies and Complement Activity Determinations in Pig to Primate XenotransplantationNieves Doménech 6. Studies on Coagulation Incompatibilities for XenotransplantationCristiana Bulato, Claudia Radu, Paolo Simioni7. Cellular Studies for in Vitro Modeling of Xenogeneic Immune ResponsesRoberta Sommaggio, Magdiel Pérez-Cruz, and Cristina Costa 8. Production of Transgenic and Knock Out Pigs by Somatic Cell Nuclear TransferAngelica M. Giraldo, Suyapa Ball, Kenneth R. Bondioli9. Small Animal Models of XenotransplantationHao Wang10. Heart Xenotransplantation in Primate ModelsJohannes Postrach, Andreas Bauer, Michael Schmoeckel,  Bruno Reichart, and Paolo Brenner11. Xenogeneic Lung Transplantation ModelsLars Burdorf, Agnes M. Azimzadeh and Richard N. Pierson III12. Thymic Transplantation in Pig-to-nonhuman Primates for the Induction of Tolerance Across Xenogeneic BarriersKazuhiko Yamada and Joseph Scalea 13. Isolation of Porcine Pancreatic Islets for XenotransplantationKarin Ulrichs, Sibylle Eber, Bianca Schneiker, Sabine Gahn, Armin Strauß, Vasiliy Moskalenko, Irina Chodnevskaja 14. Pig Neural Cells Derived from Foetal Mesencephalon as Cell Source for Intracerebral XenotransplantationXavier Lévêque, Véronique Nerrière-Daguin, Isabelle Neveuand Philippe Naveilhan15. Hepatocyte Xenotransplantation  Katia R. F. Lima-Quaresma, Andre Gustavo Bonavita, Matheus Kafuri Cytrangulo, Marcelo Alves Pinto, and Luiz Anastácio Alves 16. In Vitro Repair Model of Focal Articular Cartilage Defects in HumansDíaz Prado SM, Fuentes-Boquete IM, Blanco FJ  17. Potential Zoonotic Infection of PERV in Xenotransplantation Giada Mattiuzzo, Yashuhiro Takeuchi, Linda Scobie 18. Ethical and Regulatory Issues for Clinical Trials in XenotransplantationJorge Guerra González 19. Some Ethical, Social and Legal Considerations of Xenotransplantation María Jorqui Azofra  and Carlos María Romeo Casabona>