The discipline of developmental toxicology is an integration of concepts, models, and methodologies based heavily on the superimposition of toxicology principles upon the science of developmental biology.  The science of developmental toxicology also borrows from other research areas that are concerned with regulation of cell growth, migration, differentiation and cell death, as such are central to the study of stem cells, cancer, and chronic diseases. In Developmental Toxicology: Methods and Protocols expert researchers in the field detail many of the methods which are now commonly used to study developmental toxicology highlighting the evolution of methods from classical teratology approaches to the dynamic, state-of-the-art molecular methods, systems biology, and next generation models and procedures. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Developmental Toxicology: Methods and Protocols is a valuable resource to those planning experiments to investigate consequences of environmental, nutritional, or chemical effects caused during development.

Inhaltsangabe1. Volume Overview - IntroductionCraig Harris 2. In Vivo Models of Developmental ToxicologyJason M. Hansen 3. Caenorhabditis Elegans as a Model in Developmental ToxicologyWindy A. Boyd, Marjolein V. Smith, and Jonathan H. Freedman 4. Zebrafish Embryo Developmental Toxicology AssayJulieta M. Panzica-Kelly, Cindy X. Zhang, Karen Augustine-Rauch 5. Gene Knockdown by Morpholino-modified Oligonucleotides in the Zebrafish (Danio rerio) Model: Applications for Developmental Toxicology Alicia R. Timme-Laragy, Sibel I. Karchner, Mark E. Hahn 6. An Amphibian Model for Studies of Developmental Reproductive Toxicity Cecilia Berg 7. Avian Models in Teratology and Developmental ToxicologySusan M. Smith, George R. Flentke, Ana Garic 8. Overview of In Vitro Models in Developmental ToxicologyCraig Harris 9. Primary Cell and Micromass Culture in Assessing Developmental ToxicityM Pratten, Bhavesh K. Ahir, H. Smith-Hurst, S Memon, P Mutch & P.Cumberland 10. Embryonic Stem Cell Test (EST): Stem cell Use in Predicting Developmental Cardiotoxicity and OsteotoxicityBéatrice Kuske, Polina Y. Pulyanina, Nicole I. zur Nieden 11. Mouse Embryonic Stem Cell Adherent Cell Differentiation and Cytotoxicity (ACDC) AssayMarianne Barrier, Kelly Chandler, Susan Jeffay, Maria Hoopes, Tom Knudsen, Sid Hunter 12. The Murine Limb Bud in Culture as an In Vitro Teratogenicity Test SystemFrance-Hélène Paradis, Chunwei Huang and Barbara F. Hales 13. Rodent Whole Embryo CultureCraig Harris 14. Rabbit Whole Embryo CultureValeris A. Marshall and Edward W. Carney 15. Assessment of Xenobiotic Biotransformation Including Reactive Oxygen Species Generation in the Embryo using Benzene as an ExampleHelen J. Renaud, Allison Rutter, and Louise M. Winn 16. Methodological Approaches to Cytochrome P450 Profiling in EmbryosJared V. Goldstone and John J. Stegeman 17. Analysis of Nrf2-Mediated Transcriptional Induction of Antioxidant Response in Early EmbryosShao-yu Chen 18. Regulation and Control of AP-1 Binding Activity in EmbryotoxicityTerence R.S. Ozolins 19. Thioredoxin Redox Status Assessment During Embryonic Development:  The Redox WesternJason M. Hansen 20. Methods for the Determination of Plasma or Tissue Glutathione LevelsTrent E. Tipple and Lynette K. Rogers21. Oxidative Stress, Thiols, and Redox Profiles Craig Harris and Jason M. Hansen22. A Review of Toxicogenomic Approaches in Developmental ToxicologyJoshua F. Robinson, Jeroen L.A. Pennings, Aldert H. Piersma23. Epigenetic Approaches and Methods in Developmental Toxicology: Role of Inhibition in Teratogenic EventsElena Menegola, Graziella Cappelletti and Francesca Di Renzo24. DNA Methylation Screening and AnalysisKarilyn E. Sant, Muna S. Nahar, and Dana C. Dolinoy25. Assessment of Histiotrophic Nutrition using Fluorescent ProbesJeffrey Ambroso and Craig Harris26. Diabetic EmbryopathyUlf J. Eriksson and Parri Wentzel 27. Gene Expression Analysis in Developing Embryos: In situ HybridizationSiew-Ging Gong 28. Assessment of Gross Fetal Malformations:  The Modernized Wilson Technique and Skeletal StainingBRobert E. Seegmiller, Nathan Cook, Korance Goodwin, and Timothy Leishman>