Oral health is the pathway to ones general health and wellness. Mouthwash is a product used as an adjunct to oral hygiene practices as an antiseptic and antiplaque and antimicrobial agent. The awareness of using mouthwash solution is so limited worldwide. And maybe theres an effect of mouthwash on commensal flora. The aim of this study is to measure the effect of using Mouthwash solution on the commensal flora of the oral cavity. In this study, we determined three groups, the first group is considered as a basic group where Female students are not using any mouthwash, the second group used mouthwash twice daily for two weeks, group number three used mouthwash twice weekly. We take a cytological sample scraping from the oral cavity. All the cytological sections of all groups stained with Papanicolaou stain and examined the slides under the microscope. The study came out with the following results: The basic group that did not use any mouthwash remained unchanged, while the second group, who used mouthwash twice daily, showed a clear epithelial tissue for any bacteria and a clear background. Group number three, who used mouthwash twice a week, showed countable bacteria in epithelial

1- Dr. Amal Hussain Mohammed AliAssistant Professor-Histopathology &Cytology.Department of Medical LaboratoriesCollege of Applied Medical SciencesQassim University 2- Shaden Mohammed AlhathloulMedical  Laboratories StudentQassim University, Saudi Arabi