Telomerase Inhibition

Strategies and Protocols, Methods in Molecular Biology 405

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Bibliografische Daten
ISBN/EAN: 9781588296832
Sprache: Englisch
Umfang: x, 193 S.
Auflage: 1. Auflage 2007
Einband: gebundenes Buch

Beschreibung

InhaltsangabeContents: Preface Contributors 1. Methods of Telomerase Inhibition Lucy G. Andrews and Trygve O. Tollefsbol 2. Telomerase Inhibition by Synthetic Nucleic Acids and Chemosensitization in Human Bladder Cancer Cell Lines Kai Kraemer, Susanne Fuessel, and Axel Meye 3. hTERT Knockdown in Human Embryonic Kidney Cells Using DoubleStranded RNA Serene R. Lai, Lucy G. Andrews, and Trygve O. Tollefsbol 4. RNA Interference Using a Plasmid Construct Expressing Short Hairpin RNA Serene R. Lai, Lucy G. Andrews, and Trygve O. Tollefsbol 5. Retrovirus-mediated RNA Interference: Targeting hTERT Through Stable Expression of Short Hairpin RNA Amanda P. Cunningham, Lucy G. Andrews, and Trygve O. Tollefsbol 6. Telomerase Inhibition and Telomere Targeting in Hematopoietic Cell Lines with Small Non-Nucleosidic Synthetic Compounds (BIBR1532) Hesham El Daly and Uwe M. Martens 7. Uses of Telomerase Peptides in Anti-tumor Immune Therapy He Li, Indzi Katik, and Jun-Ping Liu 8. Establishing Cell-based Reporter Systems for the Analysis of hTERT expression YiYuan Huang, JingWen Shih, and JingJer Lin 9. Telomerase RNA Inhibition Using Antisense Oligonucleotide Against Human Telomerase RNA Linked to a 2', 5'-Oligoadenylate (2-5A) Yasuko Kondo and Seji Kondo 10. Knockdown of Telomerase RNA Using Hammerhead Ribozymes and RNA Interference Shang Li, Mehdi Nosrati, and Mohammed Kashani-Sabet 11. Evaluation of Tankyrase Inhibition in Whole Cells Tomohazu Ohishi, Takashi Tsuruo, and Hiroyuki Seimiya 12. Inhibition of Telomerase by Targeting MAP Kinase Signaling Dakang Xu, He Li, and Jun-Ping Li 13. Screening of Telomerase Inhibitors Elke Kleideiter, Kamilla Piotrowska, and Ulrich Klotz 14. Telomerase Inhibition Combined with Other Chemotherapeutic Reagents to Enhance Anti-Cancer Effect Tetsuzo Tauchi, Junko H. Ohyashiki,and Kazuma Ohyashiki

Autorenportrait

InhaltsangabeContents: Preface Contributors 1. Methods of Telomerase Inhibition Lucy G. Andrews and Trygve O. Tollefsbol 2. Telomerase Inhibition by Synthetic Nucleic Acids and Chemosensitization in Human Bladder Cancer Cell Lines Kai Kraemer, Susanne Fuessel, and Axel Meye 3. hTERT Knockdown in Human Embryonic Kidney Cells Using DoubleStranded RNA Serene R. Lai, Lucy G. Andrews, and Trygve O. Tollefsbol 4. RNA Interference Using a Plasmid Construct Expressing Short Hairpin RNA Serene R. Lai, Lucy G. Andrews, and Trygve O. Tollefsbol 5. Retrovirus-mediated RNA Interference: Targeting hTERT Through Stable Expression of Short Hairpin RNA Amanda P. Cunningham, Lucy G. Andrews, and Trygve O. Tollefsbol 6. Telomerase Inhibition and Telomere Targeting in Hematopoietic Cell Lines with Small Non-Nucleosidic Synthetic Compounds (BIBR1532) Hesham El Daly and Uwe M. Martens 7. Uses of Telomerase Peptides in Anti-tumor Immune Therapy He Li, Indzi Katik, and Jun-Ping Liu 8. Establishing Cell-based Reporter Systems for the Analysis of hTERT expression YiYuan Huang, JingWen Shih, and JingJer Lin 9. Telomerase RNA Inhibition Using Antisense Oligonucleotide Against Human Telomerase RNA Linked to a 2', 5'-Oligoadenylate (2-5A) Yasuko Kondo and Seji Kondo 10. Knockdown of Telomerase RNA Using Hammerhead Ribozymes and RNA Interference Shang Li, Mehdi Nosrati, and Mohammed Kashani-Sabet 11. Evaluation of Tankyrase Inhibition in Whole Cells Tomohazu Ohishi, Takashi Tsuruo, and Hiroyuki Seimiya 12. Inhibition of Telomerase by Targeting MAP Kinase Signaling Dakang Xu, He Li, and Jun-Ping Li 13. Screening of Telomerase Inhibitors Elke Kleideiter, Kamilla Piotrowska, and Ulrich Klotz 14. Telomerase Inhibition Combined with Other Chemotherapeutic Reagents to Enhance Anti-Cancer Effect Tetsuzo Tauchi, Junko H. Ohyashiki,and Kazuma Ohyashiki